Antifungal Activity of α-Sarcin against Penicillium digitatum: Proposal of a New Role for Fungal Ribotoxins,ACS Chemical Biology

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Antifungal Activity of α-Sarcin against Penicillium digitatum: Proposal of a New Role for Fungal Ribotoxins
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2018-06-28 00:00:00 , DOI: 10.1021/acschembio.8b00410
Lucía Citores ₁ , Rosario Iglesias ₁ , Sara Ragucci ₂ , Antimo Di Maro ₂ , José M. Ferreras ₁

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Among the putative defense proteins that occur in fungi, one of the best studied is α-sarcin, produced by the mold Aspergillus giganteus. This protein is the most significant member of the ribotoxin family, which consists of extracellular rRNA ribonucleases that display cytotoxic activity toward animal cells. Ribotoxins are rRNA endonucleases that catalyze the hydrolysis of the phosphodiester bond between G4325 and A4326 from the rat 28S rRNA. The results of several experimental approaches have led to propose ribotoxins as insecticidal agents. In this work, we report that α-sarcin displays a strong antifungal activity against Penicillium digitatum, being able to enter into the cytosol where it inactivates the ribosomes, thus killing the cells and arresting the growth of the fungus. This is the first time that a ribotoxin has been found to display antifungal activity. Therefore, this protein could play, besides the already proposed insecticidal function, a role in nature as an antifungal agent.

中文翻译：

α-Sarcin对趾状青霉的抗真菌活性：真菌利博毒素的新作用的建议。

在真菌中存在的推定防御蛋白中，研究最深入的蛋白之一是由霉菌曲霉（Aspergillus giganteus）产生的α-sarcin 。该蛋白是核毒素家族中最重要的成员，它由对动物细胞表现出细胞毒活性的细胞外rRNA核糖核酸酶组成。核糖蛋白是rRNA内切酶，可催化大鼠28S rRNA水解G4325和A4326之间的磷酸二酯键。几种实验方法的结果导致提出了核糖毒素作为杀虫剂的提议。在这项工作中，我们报告说，α-sarcin对数指青霉有较强的抗真菌活性。能够进入细胞质，使核糖体失活，从而杀死细胞并阻止真菌的生长。这是第一次发现核糖毒素显示出抗真菌活性。因此，除了已经提出的杀虫功能之外，该蛋白质还可以在自然界中起到抗真菌剂的作用。

更新日期：2018-06-28

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