Abstract The Ultraviolet-C technology is proposed as an alternative to thermal pasteurization for decontamination of soymilk, as heat treatments may adversely affect its quality. The aim of this study was to evaluate and model the influence of different processing temperatures (4–30 °C) on the inactivation of Salmonella Enteritidis in soymilk at different UV-C doses. Inactivation efficiency was potentiated by the increase in temperature from 4 to 18 °C. Higher temperatures (ranging from 18 to 30 °C) did not yield significant differences between Salmonella maximum inactivation levels, i.e. difference between concentrations after and before treatments. The Weibull model was suitable to describe the inactivation data as a function of UV-C dose, at all temperatures evaluated. Since the Weibull shape parameter (p) was statistically homogeneous at temperatures ranging from 8 to 30 °C (P > 0.05), it was fixed at its mean value (0.68 ± 0.08) and inactivation curves were re-fitted. Secondary modelling was conducted only to establish a relationship between Weibull model scale parameter (δ) and temperature. The δ estimations indicate that lower UV-C doses are required at 18–30 °C to attain a first tenfold reduction of Salmonella population in soymilk. Finally, a model combining primary and secondary models was developed to predict Salmonella inactivation at different UV-C doses and temperatures. This model provided acceptable predictions of inactivation data at temperatures within its limits. Accurate predictions of microbial inactivation curves at different combinations of UV-C doses and processing temperatures would be beneficial in setting favourable conditions of UV-C application to achieve desired levels of bacterial inactivation in soymilk.

中文翻译：

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UV-C技术在豆浆中应用温度对肠炎沙门氏菌灭活动力学的影响

摘要 紫外线-C 技术被提议作为热巴氏杀菌的替代方法，用于豆浆的去污，因为热处理可能对其质量产生不利影响。本研究的目的是评估和模拟不同加工温度 (4–30 °C) 在不同 UV-C 剂量下对豆奶中肠炎沙门氏菌灭活的影响。温度从 4°C 增加到 18°C 会增强灭活效率。较高的温度（范围从 18 到 30 °C）不会产生沙门氏菌最大灭活水平之间的显着差异，即处理前后浓度之间的差异。Weibull 模型适用于在所有评估温度下将灭活数据描述为 UV-C 剂量的函数。由于威布尔形状参数 (p) 在 8 到 30 °C (P > 0.05) 的温度范围内在统计上是均匀的，因此固定为其平均值 (0.68 ± 0.08) 并重新拟合灭活曲线。进行二次建模只是为了建立威布尔模型尺度参数 (δ) 和温度之间的关系。δ 估计值表明，在 18-30 °C 时需要较低的 UV-C 剂量才能使豆奶中的沙门氏菌数量减少 10 倍。最后，开发了一个结合初级和次级模型的模型来预测不同 UV-C 剂量和温度下沙门氏菌的灭活。该模型在其限值内的温度下提供了可接受的失活数据预测。