Rapid and Versatile Cell Aggregate Formation Using Lipid-Conjugated Heparin,ACS Applied Materials & Interfaces

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Rapid and Versatile Cell Aggregate Formation Using Lipid-Conjugated Heparin
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-06-28 00:00:00 , DOI: 10.1021/acsami.8b07731
Eunsol Kim ₁ , Jong Chul Kim ₁ , Kiyoon Min ₁ , MeeiChyn Goh ₁ , Giyoong Tae ₁

Affiliation

Cell aggregates hold significant therapeutic promise for in vitro cell analysis, ex vivo tissue models, and in vivo cell therapy and tissue engineering. Traditional methods of making cell aggregates require long incubation times and can only produce three-dimensional-spheroid-shaped aggregates. We propose a novel method of making cell aggregates of diverse sizes and shapes using lipid-conjugated heparin. Shaking the cell suspension containing a small amount of lipid-conjugated heparin for approximately 30 min produced cell aggregates. This approach can be applied to any cell type, including stem cells, fibroblast cells, and T lymphocytes. The shape of biocompatible templates could modulate the shape of cell aggregates. In addition to layered, multicompartmental cell aggregates on template, template-free, tube-shaped cell aggregates could also be made. The cell aggregates formed were alive and maintained biological activities.

中文翻译：

使用脂质结合的肝素快速而多功能的细胞聚集体形成

细胞聚集体对于体外细胞分析，离体组织模型以及体内细胞治疗和组织工程具有重要的治疗前景。制备细胞聚集体的传统方法需要较长的孵育时间，并且只能产生三维球形的聚集体。我们提出了一种使用脂质结合的肝素制备各种大小和形状的细胞聚集体的新方法。摇动含有少量脂质结合肝素的细胞悬液约30分钟，产生细胞聚集体。该方法可以应用于任何细胞类型，包括干细胞，成纤维细胞和T淋巴细胞。生物相容性模板的形状可以调节细胞聚集体的形状。除了模板上的分层，多隔室单元格聚合，无模板，也可以制成管状细胞聚集体。形成的细胞聚集体是活的并保持生物学活性。

更新日期：2018-06-28

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