Orientation Control of Trametes Laccases on a Carbon Electrode Surface to Understand the Orientation Effect on the Electrocatalytic Activity,Bioconjugate Chemistry

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Orientation Control of Trametes Laccases on a Carbon Electrode Surface to Understand the Orientation Effect on the Electrocatalytic Activity
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-06-28 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00344
Yasuto Hoshikawa ₁ , Alberto Castro-Muñiz ₁ , Hanako Tawata ₁ , Kouichi Nozaki ₂ , Shohei Yamane ₂ , Tetsuji Itoh ₃ , Takashi Kyotani ₁

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By using a carbon-coated anodic aluminum oxide (CAAO) film as a monolithic porous electrode for the immobilization of Trametes laccases (LACs), an attempt is made to control the orientation of LAC molecules toward the electrode surface simply by applying an electric potential to the CAAO film. Because the resulting film is characterized by a myriad of open, simple, and straight nanochannels with diameters as large as 40 nm, the O₂ diffusion problem in pores is minimized, thereby making it possible to highlight the effect of such orientation on the electrocatalytic activity as a biocathode. It has been evidenced that LAC molecules are favorably oriented for a smooth electron transfer from the electrode when the LACs are immobilized with applying a positive voltage to the electrode, and such favorable orientation exhibits 3.7-times higher electrocatalytic activity than unfavorable orientation. Furthermore, the orientation mechanism has been rationally explained in terms of local surface chemistry on a LAC molecule.

中文翻译：

碳电极表面上的孔雀菌漆酶的取向控制，以了解取向对电催化活性的影响

通过使用碳包覆的阳极氧化铝（CAAO）膜作为固定Trametes漆酶（LAC）的整体多孔电极，尝试通过简单地施加电势来控制LAC分子朝向电极表面的取向。 CAAO电影。由于所得膜的特征是直径大至40 nm的无数个开放，简单和直的纳米通道，因此O ₂孔中的扩散问题被最小化，从而使得有可能突出这种取向对作为生物阴极的电催化活性的影响。已经证明，当通过在电极上施加正电压而固定LAC时，LAC分子有利于从电极进行平稳的电子转移，并且这种有利的取向表现出比不利的取向高3.7倍的电催化活性。此外，已经根据LAC分子上的局部表面化学合理地解释了取向机理。

更新日期：2018-06-28

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