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Surface-initiated atom transfer radical polymerization of cationic corona on iron oxide nanoparticles for magnetic sorting of macrophages†
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-06-28 00:00:00 , DOI: 10.1039/c8bm00418h Myun Koo Kang 1, 2, 3, 4 , Wei Mao 1, 2, 3, 4 , Hyuk Sang Yoo 1, 2, 3, 4, 5
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-06-28 00:00:00 , DOI: 10.1039/c8bm00418h Myun Koo Kang 1, 2, 3, 4 , Wei Mao 1, 2, 3, 4 , Hyuk Sang Yoo 1, 2, 3, 4, 5
Affiliation
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Ovalbumin-incorporated antigen carriers were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) of iron oxide nanoparticles for magnetic sorting of antigen-presenting cells. Iron oxide nanoparticles were surface-decorated with cationic shells by SI-ATRP, and the primary amines on the surface were further tri-methylated. Surface decoration of the nanoparticles was characterized by Fourier transform infrared spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and transmission electron microscopy with energy-dispersive spectrometry. Ovalbumin-loaded nanoparticles showed higher incorporation in comparison to non-decorated nanoparticles, and the loaded ovalbumin was released faster at low pH than at neutral pH. Ovalbumin-loaded nanoparticles were endocytosed by macrophages, and macrophages with nanoparticles were easily harvested by magnetic separation. Magnetically sorted macrophages showed higher release of cytokines including TNF-α, MIP-1α, and IL-1β than unsorted macrophages. These results suggest that ovalbumin-loaded nanoparticles can potentially increase the efficiency of immune therapy during the antigen-presenting pathway.
中文翻译：
阳离子电晕在氧化铁纳米颗粒上的表面引发的原子转移自由基聚合，用于巨噬细胞的磁性分选†
通过表面引发的氧化铁纳米粒子的原子引发自由基转移自由基聚合（SI-ATRP）来制备掺入卵白蛋白的抗原载体，以对抗原呈递细胞进行磁性分选。通过SI-ATRP用阳离子壳对氧化铁纳米颗粒进行表面修饰，然后将表面上的伯胺进一步进行三甲基化。纳米粒子的表面装饰通过傅里叶变换红外光谱，基质辅助激光解吸/电离飞行时间质谱和能量分散透射电子显微镜进行表征。与未装饰的纳米颗粒相比，负载卵白蛋白的纳米颗粒显示出更高的结合度，并且负载的卵白蛋白在低pH下的释放速度要快于中性pH。卵清蛋白负载的纳米颗粒被巨噬细胞内吞，磁分离法很容易收集到带有纳米颗粒的巨噬细胞。磁性分选的巨噬细胞显示出比未分选的巨噬细胞更高的细胞因子释放，包括TNF-α，MIP-1α和IL-1β。这些结果表明，装载卵白蛋白的纳米颗粒可以潜在地提高抗原呈递途径中的免疫治疗效率。
更新日期：2018-06-28
中文翻译：
阳离子电晕在氧化铁纳米颗粒上的表面引发的原子转移自由基聚合，用于巨噬细胞的磁性分选†
通过表面引发的氧化铁纳米粒子的原子引发自由基转移自由基聚合（SI-ATRP）来制备掺入卵白蛋白的抗原载体，以对抗原呈递细胞进行磁性分选。通过SI-ATRP用阳离子壳对氧化铁纳米颗粒进行表面修饰，然后将表面上的伯胺进一步进行三甲基化。纳米粒子的表面装饰通过傅里叶变换红外光谱，基质辅助激光解吸/电离飞行时间质谱和能量分散透射电子显微镜进行表征。与未装饰的纳米颗粒相比，负载卵白蛋白的纳米颗粒显示出更高的结合度，并且负载的卵白蛋白在低pH下的释放速度要快于中性pH。卵清蛋白负载的纳米颗粒被巨噬细胞内吞，磁分离法很容易收集到带有纳米颗粒的巨噬细胞。磁性分选的巨噬细胞显示出比未分选的巨噬细胞更高的细胞因子释放，包括TNF-α，MIP-1α和IL-1β。这些结果表明，装载卵白蛋白的纳米颗粒可以潜在地提高抗原呈递途径中的免疫治疗效率。
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