Metabolome Profiling of Fish Muscle Tissue Exposed to Benzo[a]pyrene Using in Vivo Solid-Phase Microextraction,Environmental Science & Technology Letters

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Metabolome Profiling of Fish Muscle Tissue Exposed to Benzo[a]pyrene Using in Vivo Solid-Phase Microextraction
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2018-06-28 , DOI: 10.1021/acs.estlett.8b00272
Anna Roszkowska ₁ , Miao Yu ₁ , Vincent Bessonneau ₁ , Leslie Bragg ₂ , Mark Servos ₂ , Janusz Pawliszyn ₁

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In vivo solid-phase microextraction (SPME) of endogenous compounds may depict changes in the biochemical pathways in organisms that have been affected by various stimuli such as environmental pollutants. In this paper, we use low-invasive in vivo SPME to analyze metabolic profile changes in the muscle tissue of living fish that have been exposed to benzo[a]pyrene (BaP). This study employs a case-control model with the fish divided into three groups: a control group, a low-dose group (1 ng/L BaP exposure), and a high-dose group (10 ng/L BaP exposure). SPME probes were used to analyze the effects of short-term (after 1 day) and long-term (after 14 days) exposure to the pollutant. SPME analysis of dose-dependent and time-dependent differences in metabolome between the groups revealed distinct BaP-altered signaling pathways, mainly involving amino acids, lipids, and the components of osmotic regulation in fish. However, after 14 days, the metabolic profile of the low-dose group more closely resembled the profile of the control group than it did the high-dose group. SPME provided important information about the exposome and potential biomarkers related to BaP exposure and could be used as a powerful tool for direct monitoring of the toxicological effects of contaminants in living systems.

中文翻译：

体内固相微萃取暴露于苯并[ a ] re的鱼肌肉组织的代谢组分析

内源性化合物的体内固相微萃取（SPME）可能描述了受各种刺激（例如环境污染物）影响的生物体内生化途径的变化。在本文中，我们使用低侵入性体内SPME分析了暴露于苯并[ a]的活鱼的肌肉组织中的代谢谱变化。py（BaP）。本研究采用病例对照模型，将鱼分为三组：对照组，低剂量组（1 ng / L BaP暴露量）和高剂量组（10 ng / L BaP暴露量）。使用SPME探针分析污染物短期（1天后）和长期（14天后）的影响。两组之间代谢组的剂量依赖性和时间依赖性差异的SPME分析揭示了BaP改变的独特信号通路，主要涉及氨基酸，脂质和鱼类渗透调节成分。但是，在14天后，低剂量组的代谢特征与高剂量组的代谢特征更接近于对照组。

更新日期：2018-06-30

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