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A fluorometric skin-interfaced microfluidic device and smartphone imaging module for in situ quantitative analysis of sweat chemistry†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-06-29 00:00:00 , DOI: 10.1039/c8lc00530c Yurina Sekine 1, 2, 3, 4 , Sung Bong Kim 5, 6, 7, 8, 9 , Yi Zhang 5, 9, 10, 11, 12 , Amay J. Bandodkar 5, 9, 10, 11, 12 , Shuai Xu 9, 10, 11, 12, 13 , Jungil Choi 5, 9, 10, 11, 12 , Masahiro Irie 4, 14, 15, 16 , Tyler R. Ray 5, 9, 10, 11, 12 , Punit Kohli 9, 17, 18, 19 , Naofumi Kozai 2, 3, 4, 20 , Tsuyoshi Sugita 2, 3, 4, 20 , Yixin Wu 5, 9, 10, 11 , KunHyuck Lee 5, 9, 10, 11 , Kyu-Tae Lee 21, 22, 23, 24 , Roozbeh Ghaffari 9, 10, 11, 12, 13 , John A. Rogers 5, 6, 7, 8, 9
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-06-29 00:00:00 , DOI: 10.1039/c8lc00530c Yurina Sekine 1, 2, 3, 4 , Sung Bong Kim 5, 6, 7, 8, 9 , Yi Zhang 5, 9, 10, 11, 12 , Amay J. Bandodkar 5, 9, 10, 11, 12 , Shuai Xu 9, 10, 11, 12, 13 , Jungil Choi 5, 9, 10, 11, 12 , Masahiro Irie 4, 14, 15, 16 , Tyler R. Ray 5, 9, 10, 11, 12 , Punit Kohli 9, 17, 18, 19 , Naofumi Kozai 2, 3, 4, 20 , Tsuyoshi Sugita 2, 3, 4, 20 , Yixin Wu 5, 9, 10, 11 , KunHyuck Lee 5, 9, 10, 11 , Kyu-Tae Lee 21, 22, 23, 24 , Roozbeh Ghaffari 9, 10, 11, 12, 13 , John A. Rogers 5, 6, 7, 8, 9
Affiliation
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The rich composition of solutes and metabolites in sweat and its relative ease of collection upon excretion from skin pores make this class of biofluid an attractive candidate for point of care analysis. Wearable technologies that combine electrochemical sensors with conventional or emerging semiconductor device technologies offer valuable capabilities in sweat sensing, but they are limited to assays that support amperometric, potentiometric, and colorimetric analyses. Here, we present a complementary approach that exploits fluorometric sensing modalities integrated into a soft, skin-interfaced microfluidic system which, when paired with a simple smartphone-based imaging module, allows for in situ measurement of important biomarkers in sweat. A network array of microchannels and a collection of microreservoirs pre-filled with fluorescent probes that selectively react with target analytes in sweat (e.g. probes), enable quantitative, rapid analysis. Field studies on human subjects demonstrate the ability to measure the concentrations of chloride, sodium and zinc in sweat, with accuracy that matches that of conventional laboratory techniques. The results highlight the versatility of advanced fluorescent-based imaging modalities in body-worn sweat microfluidics platforms, and they suggest some practical potential for these ideas.
中文翻译：
荧光的皮肤界面微流控设备和智能手机成像模块，用于汗液化学原位定量分析†
汗液中溶质和代谢物的丰富组成以及从皮肤毛孔排泄后相对容易收集，使此类生物流体成为护理点分析的有吸引力的候选对象。将电化学传感器与常规或新兴的半导体器件技术相结合的可穿戴技术在汗液感测中提供了宝贵的功能，但仅限于支持电流分析，电位分析和比色分析的测定法。在这里，我们提出了一种补充方法，该方法利用了集成到柔软的皮肤界面微流体系统中的荧光传感模式，当与基于智能手机的简单成像模块配合使用时，就可以进行原位检测汗液中重要生物标志物的检测。微通道的网络阵列和预填充有荧光探针的微容器集合，荧光探针可以与汗液中的目标分析物（例如探针）选择性地反应，从而可以进行定量，快速的分析。对人类受试者的野外研究证明了测量汗液中氯，钠和锌浓度的能力，其准确性与传统实验室技术相当。结果强调了在人体佩戴的汗液微流体平台中先进的基于荧光的成像方法的多功能性，并为这些想法提出了一些实际的潜力。
更新日期：2018-06-29
中文翻译：
荧光的皮肤界面微流控设备和智能手机成像模块，用于汗液化学原位定量分析†
汗液中溶质和代谢物的丰富组成以及从皮肤毛孔排泄后相对容易收集，使此类生物流体成为护理点分析的有吸引力的候选对象。将电化学传感器与常规或新兴的半导体器件技术相结合的可穿戴技术在汗液感测中提供了宝贵的功能，但仅限于支持电流分析，电位分析和比色分析的测定法。在这里，我们提出了一种补充方法，该方法利用了集成到柔软的皮肤界面微流体系统中的荧光传感模式，当与基于智能手机的简单成像模块配合使用时，就可以进行原位检测汗液中重要生物标志物的检测。微通道的网络阵列和预填充有荧光探针的微容器集合，荧光探针可以与汗液中的目标分析物（例如探针）选择性地反应，从而可以进行定量，快速的分析。对人类受试者的野外研究证明了测量汗液中氯，钠和锌浓度的能力，其准确性与传统实验室技术相当。结果强调了在人体佩戴的汗液微流体平台中先进的基于荧光的成像方法的多功能性，并为这些想法提出了一些实际的潜力。
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