Multiple microarrays of non-adherent cells on a single 3D stimuli-responsive binary polymer-brush pattern†,Journal of Materials Chemistry B

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Multiple microarrays of non-adherent cells on a single 3D stimuli-responsive binary polymer-brush pattern†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-06-28 00:00:00 , DOI: 10.1039/c8tb01441h
Jianwen Hou _{1,

2,

3,

4,

5} , Runhai Chen _{1,

2,

3,

4,

5} , Jingchuan Liu _{1,

2,

3,

4,

5} , Haozheng Wang _{1,

2,

3,

4,

5} , Qiang Shi _{1,

2,

3,

4,

5} , Zhirong Xin _{5,

6,

7,

8,

9} , Shing-Chung Wong _{10,

11,

12,

13} , Jinghua Yin _{1,

2,

3,

4,

5}

Affiliation

We have developed a 3D smart binary polymer-brush pattern on the polymer substrate for inducing multiple cell microarrays aided by a lectin and temperature. The binary polymer-brush pattern composed of poly(N-isopropylacrylamide) (PNIPAM) and poly(D-gluconamidoethyl methacrylate) (PGAMA) brushes is fabricated by combining the photolithography technique with a surface-initiated photo-polymerization (SIPP) method. We demonstrate that well-defined binary polymer-brush patterns with high resolution are fabricated using this facile method. The patterned hierarchical PNIPAM brushes exhibit reversible switching to the adhesion of red blood cells (RBCs) induced by thermo-responsiveness. The PGAMA brush domains resist adhesion of RBCs but bind specifically with concanavalin A (Con A), forming a lectin pattern to capture RBCs in a site-specific manner. Therefore, multiple cell microarrays on a single platform are generated with the aid of Con A and temperature. This novel platform composed of a smart binary polymer-brush pattern is versatile and specific, which opens up pathways to potential applications such as microsensors, biochips and bioassays.

中文翻译：

单个3D刺激响应性二进制聚合物刷模式上的多个非贴壁细胞微阵列†

我们已经在聚合物基质上开发了3D智能二元聚合物画笔图案，以通过凝集素和温度诱导多个细胞微阵列。聚（N-异丙基丙烯酰胺）（PNIPAM）和聚（D通过将光刻技术与表面引发的光致聚合（SIPP）方法相结合，制造了（甲基丙烯酸-葡糖酰胺基乙基甲基丙烯酸酯）（PGAMA）刷子。我们证明了使用这种简便的方法可以制造出具有高分辨率的清晰定义的二元聚合物画笔图案。图案化的分层PNIPAM画笔显示出可逆切换到由热响应性诱导的红细胞（RBC）粘附。PGAMA刷结构域可抵抗RBC的粘附，但可与伴刀豆球蛋白A（Con A）特异性结合，形成凝集素模式，以位点特异性方式捕获RBC。因此，借助Con A和温度可在单个平台上生成多个细胞微阵列。这个由智能二进制聚合物画笔模式组成的新颖平台具有多种用途和特定功能，

更新日期：2018-06-28

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