Cone Snail Glutaminyl Cyclase Sequences from Transcriptomic Analysis and Mass Spectrometric Characterization of Two Pyroglutamyl Conotoxins,Journal of Proteome Research

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Cone Snail Glutaminyl Cyclase Sequences from Transcriptomic Analysis and Mass Spectrometric Characterization of Two Pyroglutamyl Conotoxins
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-07-11 , DOI: 10.1021/acs.jproteome.8b00132
Marimuthu Vijayasarathy _{1,

2} , Soorej M. Basheer _{1,

3} , Padmanabhan Balaram _{1,

2}

Affiliation

The post-translational modification of N-terminal glutamine (Q) to a pyroglutamyl (Z) residue is observed in the conotoxins produced by marine cone snails. This conversion requires the action of the enzyme glutaminyl cyclase (QC). Four complete QC sequences from the species C. araneosus, C. frigidus, C. litteratus, and C. monile and two partial sequences from C. amadis and C. miles have been obtained by analysis of transcriptomic data. Comparisons with mammalian enzyme sequences establish a high level of identity and complete conservation of functional active site residues, including a cluster of hydrogen-bonded acidic side chains. Mass spectrometric analysis of crude venom samples coupled to conotoxin precursor protein sequences obtained from transcriptomic data establishes the presence of pyroglutamyl conotoxins in the venom of C. frigidus and C. amadis. The C. frigidus peptide belongs to the M superfamily, with cysteine framework III, whereas the C. amadis peptide belongs to the divergent superfamily with cysteine framework VI/VII. Additionally, gamma carboxylation of glutamic acid and hydroxylation of proline are observed in the C. frigidus peptide. Mass spectral data are available via ProteomeXchange with identifier PXD009006.

中文翻译：

锥虫谷氨酰胺基环化酶序列的转录组学分析和两种焦谷氨酰毒素的质谱表征

在海洋锥蜗牛产生的芋螺毒素中观察到N末端谷氨酰胺（Q）的翻译后修饰为焦谷氨酰（Z）残基。这种转化需要谷氨酰胺酰环化酶（QC）的作用。从物种四个完整的QC序列C. araneosus，C. frigidus，C. litteratus，和C. monile和从两个部分序列C.阿马迪斯和C.英里已经通过转录组数据分析获得。与哺乳动物酶序列的比较建立了高水平的同一性，并完全保留了功能性活性位点残基，包括一簇氢键结合的酸性侧链。耦合以从转录数据获得的芋螺毒素的前体蛋白序列的粗毒液的样品的质谱分析确定焦谷芋螺毒素中的毒液中存在C. frigidus和C.阿马迪斯。所述C. frigidus肽属于M个超家族，与半胱氨酸框架III，而C.阿马迪斯该肽属于具有半胱氨酸框架VI / VII的发散的超家族。此外，在C. frigidus肽中观察到了谷氨酸的γ羧基化和脯氨酸的羟基化。可以通过ProteomeXchange获得标识符为PXD009006的质谱数据。

更新日期：2018-07-12

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