Capillary electrophoresis coupled with electrochemical detection can be a powerful analysis tool; however, previous methods developed to integrate these two techniques can often times be fragile and have alignment issues such that there are no commercially available approaches. In this paper, we present the use of a 3D-printed Wall-Jet Electrode device for integrating capillary electrophoresis with electrochemical detection. A pressure mobilization step was also utilized to further reduce noise by allowing the electrophoresis separation step to continue only until the first analyte was close to elution. Then, the separation voltage was terminated and pressure-based flow was used for elution of the analyte bands onto the electrode surface with a wall-jet configuration. It is shown that the pressure-based elution is beneficial for the reduction of baseline noise and elimination of field effects. A mixture of catecholamines were separated to demonstrate effectiveness of the system. In addition, the system was coupled with a Beckman Coulter commercial capillary electrophoresis instrument in a straightforward manner. The system was also shown to be effective in separations done with a high ionic strength physiological buffer. This 3D printing approach can be used by researchers to utilize electrochemical detection on commercial capillary electrophoresis systems by downloading the provided STL and/or CAD files.

中文翻译：

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使用 3D 打印微流体装置和压力动员将毛细管电泳与电化学检测相结合

毛细管电泳与电化学检测相结合可以成为一种强大的分析工具；然而，以前为整合这两种技术而开发的方法通常很脆弱，并且存在对齐问题，因此没有商业上可用的方法。在本文中，我们介绍了使用 3D 打印壁射流电极装置将毛细管电泳与电化学检测相结合。通过允许电泳分离步骤仅持续到第一个分析物接近洗脱，压力动员步骤也被用来进一步降低噪音。然后，终止分离电压，并使用基于压力的流动将分析物带洗脱到具有壁射流配置的电极表面上。结果表明，基于压力的洗脱有利于降低基线噪声和消除场效应。分离儿茶酚胺的混合物以证明系统的有效性。此外，该系统以简单的方式与 Beckman Coulter 商用毛细管电泳仪相连。该系统还被证明在使用高离子强度生理缓冲液进行的分离中是有效的。通过下载提供的 STL 和/或 CAD 文件，研究人员可以使用这种 3D 打印方法在商业毛细管电泳系统上利用电化学检测。该系统以简单的方式与 Beckman Coulter 商用毛细管电泳仪相连。该系统还被证明在使用高离子强度生理缓冲液进行的分离中是有效的。通过下载提供的 STL 和/或 CAD 文件，研究人员可以使用这种 3D 打印方法在商业毛细管电泳系统上利用电化学检测。该系统以简单的方式与 Beckman Coulter 商用毛细管电泳仪相连。该系统还被证明在使用高离子强度生理缓冲液进行的分离中是有效的。通过下载提供的 STL 和/或 CAD 文件，研究人员可以使用这种 3D 打印方法在商业毛细管电泳系统上利用电化学检测。