Photography was employed for the quantitation and differentiation of G- and V-series nerve agent mimics with the use of self-propagating cascades. Fluoride anion and thiols, released from a G-nerve agent mimic (i.e., diisopropyl fluorophosphate) and a V-nerve agent mimic (i.e., demeton-S-methyl), respectively, were used to initiate self-propagating cascades that amplify fluorescence signals exponentially in a ratiometric manner. A homemade LEGO dark-box, a cell phone, and 96-well plates were employed to collect photographs of the fluorescence response to the analytes. The photographic images were digitally processed in the 1931 xyY color space using a watershed and morphological erosion algorithm to generate chromaticity vs concentration calibration curves. We show that the two different amplification routines are selective for their analyte class and thus successfully discriminated the G- and V-series nerve agent mimics. Further, accurate concentrations of the analytes are determined using the chromaticity and LEGO approach given herein, thus demonstrating a simple and on-site constructible/portable device for use in the field.

中文翻译：

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摄影结合自蔓延的化学级联：通过色度对G-和V-神经毒剂模拟物进行区分和定量。

使用自传播级联技术，将摄影技术用于定量和区分G系列和V系列神经毒剂模拟物。分别从G-神经毒药模拟物（即，二异丙基氟磷酸酯）和V-神经毒药模拟物（即，去甲基-S-甲基）释放的氟阴离子和硫醇用于引发自传播级联反应，从而放大荧光信号。以比例方式指数增长。使用自制的乐高黑盒，一部手机和96孔板收集对分析物的荧光反应照片。使用分水岭和形态学侵蚀算法在1931 xyY颜色空间中对照片图像进行数字处理，以生成色度与浓度的校准曲线。我们表明两种不同的扩增程序对其分析物类别具有选择性，从而成功地区分了G系列和V系列神经毒剂模拟物。此外，使用本文给出的色度和LEGO方法确定分析物的准确浓度，从而证明了在现场使用的简单和现场可构建/便携式装置。