3D Printing Nanoscale Bioactive Glass Scaffolds Enhance Osteoblast Migration and Extramembranous Osteogenesis through Stimulating Immunomodulation,Advanced Healthcare Materials

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3D Printing Nanoscale Bioactive Glass Scaffolds Enhance Osteoblast Migration and Extramembranous Osteogenesis through Stimulating Immunomodulation
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-06-27 , DOI: 10.1002/adhm.201800361
Fujian Zhao _{1,

2,

3} , Weihan Xie _{1,

2,

3} , Wen Zhang _{1,

2,

3} , Xiaoling Fu _{1,

2,

3} , Wendong Gao _{1,

2,

3} , Bo Lei ₄ , Xiaofeng Chen _{1,

2,

3}

Affiliation

Bioactive glass (BG) can repair bone defects, however, it is not clear whether BG has the ability for bone augmentation without making any bone defect. Unlike the intramembranous osteogenesis in bone defect repair, the extramembranous osteogenesis occurs outside the cortical bone and the osteoprogenitor cells show the reversed migration. Herein, nanoscale bioactive glass scaffolds (BGSs) are fabricated, and their role and immunomodulation‐related mechanism in the extramembranous osteogenesis are investigated. The in vitro migration and differentiation of calvaria preosteoblasts are studied by culturing with peripheral macrophage‐conditioned medium after stimulating with BGSs. The results indicate that the proinflammatory environment significantly promotes preosteoblast migration, but has limited effect on osteogenic differentiation. However, the anti‐inflammatory environment and BGSs significantly increase the osteogenic differentiation of preosteoblasts. The in vivo extramembranous osteogenesis evaluation shows that the active osteogenesis is observed near the skull. The osteoblasts derived from the reverse migration of cranial cells can be confirmed by comparing with the scaffolds implanted in back subcutaneous which is just colonized by fibrous tissue. This study may bring a fresh perspective for BG in bone regeneration and explore the osteogenic immunomodulation of peripheral macrophages in a nonosteogenic environment.

中文翻译：

3D 打印纳米级生物活性玻璃支架通过刺激免疫调节增强成骨细胞迁移和膜外成骨

生物活性玻璃（BG）可以修复骨缺损，但BG是否具有在不产生任何骨缺损的情况下进行骨增量的能力尚不清楚。与骨缺损修复中的膜内成骨不同，膜外成骨发生在皮质骨外，骨祖细胞显示反向迁移。在此，制备了纳米级生物活性玻璃支架（BGS），并研究了它们在膜外成骨中的作用和免疫调节相关机制。通过用 BGS 刺激后用外周巨噬细胞条件培养基培养来研究颅骨前成骨细胞的体外迁移和分化。结果表明，促炎环境显着促进前成骨细胞迁移，但对成骨分化的影响有限。然而，抗炎环境和 BGS 显着增加了前成骨细胞的成骨分化。体内膜外成骨评估表明在颅骨附近观察到活跃的成骨。通过与植入仅被纤维组织定植的背部皮下的支架进行比较，可以确认源自颅细胞反向迁移的成骨细胞。该研究可能为 BG 在骨再生中的研究带来新的视角，并探索非成骨环境中外周巨噬细胞的成骨免疫调节。通过与植入仅被纤维组织定植的背部皮下的支架进行比较，可以确认源自颅细胞反向迁移的成骨细胞。该研究可能为 BG 在骨再生中的研究带来新的视角，并探索非成骨环境中外周巨噬细胞的成骨免疫调节。通过与植入仅被纤维组织定植的背部皮下的支架进行比较，可以确认源自颅细胞反向迁移的成骨细胞。该研究可能为 BG 在骨再生中的研究带来新的视角，并探索非成骨环境中外周巨噬细胞的成骨免疫调节。

更新日期：2018-06-27

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