A split‐intein saves nine! A versatile and easy method for the rapid and high‐yield production of circularized nanodiscs for membrane scaffold protein (cMSP) research based on intein splicing in living E. coli cells is introduced. These nanodiscs offer exceptional size homogeneity and stability and are thus a robust tool for structural biology and biochemical applications.

中文翻译：

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一种基于拆分-整数的方法，可高效生产用于膜蛋白结构研究的环化纳米圆盘

拆分intein可以节省9个！介绍了一种基于活体内大肠杆菌细胞内含肽剪接的快速，高产圆环纳米膜研究的通用方法，该膜用于膜支架蛋白（cMSP）研究。这些纳米光盘具有出色的尺寸均匀性和稳定性，因此是用于结构生物学和生化应用的强大工具。