Characterization and Reshaping of a Large and Hydrophobic Nucleophile Pocket in Lipases/Acyltransferases,ChemBioChem

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Characterization and Reshaping of a Large and Hydrophobic Nucleophile Pocket in Lipases/Acyltransferases
ChemBioChem ( IF 2.6 ) Pub Date : 2018-08-08 , DOI: 10.1002/cbic.201800279
Anne‐Hélène Jan Deniau ₁ , Maeva Subileau ₁ , Eric Dubreucq ₁

Affiliation

Family characteristics: Lipases/acyltransferases catalyze acyl transfer preferentially over hydrolysis if a suitable nucleophile is present, even in a medium with a high thermodynamic activity of water. Mutations are used to investigate structural effects and reveal that the most efficient lipase/acyltransferase exhibits the largest and most hydrophobic nucleophile pocket.