RGB‐Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells,Angewandte Chemie International Edition

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RGB‐Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-07-18 , DOI: 10.1002/anie.201804304
Satoshi Arai _{1,

2,

3} , Rókus Kriszt ₄ , Kazuki Harada ₅ , Liang-Sheng Looi _{6,

7} , Shogo Matsuda ₈ , Devina Wongso ₁ , Satoshi Suo ₅ , Shoichi Ishiura ₅ , Yu-Hua Tseng ₉ , Michael Raghunath ₄ , Toshiro Ito _{6,

7} , Takashi Tsuboi _{5,

8} , Tetsuya Kitaguchi _{1,

2,

10}

Affiliation

Cell Signaling Group, Waseda Bioscience Research Institute in Singapore (WABIOS), Singapore, 138667, Republic of Singapore.
Organization for University Research Initiatives, Waseda University, Tokyo, 162-0041, Japan.
AMED-PRIME (Japan) Agency for Medical Research and Development, Tokyo, 100-0004, Japan.
Department of Biomedical Engineering, National University of Singapore, 117575, Republic of Singapore.
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, 153-8902, Japan.
Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Republic of Singapore.
Department of Biological Sciences, National University of Singapore, Singapore, 117543, Republic of Singapore.
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, 113-0033, Japan.
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, 02215, USA.
Current Address: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Kanagawa, 226-8503, Japan.

Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca²⁺ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

中文翻译：

RGB 颜色强度指示剂可可视化单细胞中 ATP 的时空动态

三磷酸腺苷 (ATP) 为生物体中多个细胞过程的调节提供能量。捕获单细胞中 ATP 的时空动态对于我们理解细胞能量代谢的机制至关重要。然而，可视化不同细胞内细胞器内和之间的 ATP 动态及其与其他信号分子的相互作用仍然具有挑战性。使用单一荧光蛋白，开发了多色 ATP 指示剂，能够同时可视化哺乳动物、植物和蠕虫细胞的细胞质和线粒体中的亚细胞 ATP 动态。此外，结合其他荧光指示剂，可以在活化的棕色脂肪细胞中观察到 ATP、cAMP 和 Ca ²⁺的动态相互作用。这套指标工具将有助于未来对能量代谢的研究。

更新日期：2018-07-18

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