In single-cell RNA sequencing (scRNA-seq) studies, only a small fraction of the transcripts present in each cell are sequenced. This leads to unreliable quantification of genes with low or moderate expression, which hinders downstream analysis. To address this challenge, we developed SAVER (single-cell analysis via expression recovery), an expression recovery method for unique molecule index (UMI)-based scRNA-seq data that borrows information across genes and cells to provide accurate expression estimates for all genes.

中文翻译：

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SAVER：基因表达恢复，用于单细胞RNA测序

在单细胞RNA测序（scRNA-seq）研究中，每个细胞中只存在一小部分的转录本被测序。这导致对具有低表达或中等表达的基因进行不可靠的定量，从而阻碍了下游分析。为了应对这一挑战，我们开发了SAVER（通过表达恢复进行单细胞分析），这是一种基于独特分子索引（UMI）的scRNA-seq数据的表达恢复方法，该方法可借用跨基因和细胞的信息来为所有基因提供准确的表达估计。