Abstract A fluorescence-quenching immunochromotographic assay (ICA) was developed for sensitive detection of aflatoxin B1 (AFB1) in soybean sauce based on the inner filter effect (IFE) between flower-like gold nanoparticles (AuNFs) and quantum dots (QDs). QDs were sprayed on the test and control line zones as background fluorescence signals, whereas AuNFs were designed as the fluorescence absorber of QDs because the surface plasma resonance peak of AuNFs totally matched with the maximum emission peak of QDs. Under the optimal conditions, the fluorescence-quenching ICA strip showed a good linear detection for AFB1 in standard AFB1 solution from 0.008 μg/L to 1 μg/L with a low detection limit of 0.004 μg/L. The average recoveries for different concentrations of AFB1-spiked soybean sauce samples ranged from 84.69% to 120.44% with a coefficient of variation ranging from 2.73% to 10.41%. In addition, the reliability of the proposed method was further confirmed by ultra-performance liquid chromatography with fluorescence detection method. In brief, this novel IFE-based strip offers a simple, rapid, sensitive, and accurate strategy for quantitative detection of AFB1 in soybean sauce.

中文翻译：

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基于内滤效应的荧光猝灭免疫层析法灵敏检测酱油中的黄曲霉毒素B 1

摘要 基于花状金纳米粒子 (AuNFs) 和量子点 (QDs) 之间的内过滤效应 (IFE)，开发了一种荧光猝灭免疫色谱法 (ICA)，用于灵敏检测酱油中的黄曲霉毒素 B1 (AFB1)。QDs 被喷洒在测试和控制线区域作为背景荧光信号，而 AuNFs 被设计为 QDs 的荧光吸收剂，因为 AuNFs 的表面等离子体共振峰与 QDs 的最大发射峰完全匹配。在最佳条件下，荧光猝灭ICA条对标准AFB1溶液中AFB1的线性检测范围为0.008 μg/L至1 μg/L，检测下限为0.004 μg/L。不同浓度的 AFB1 加标酱油样品的平均回收率为 84.69% 到 120%。44%，变异系数从 2.73% 到 10.41%。此外，采用荧光检测法的超高效液相色谱进一步证实了所提出方法的可靠性。简而言之，这种基于 IFE 的新型条带为定量检测酱油中的 AFB1 提供了一种简单、快速、灵敏和准确的策略。