Interfacing DNA with liposomes has produced a diverse range of programmable soft materials, devices, and drug delivery vehicles. By simply controlling liposomal composition, bilayer fluidity, lipid domain formation, and surface charge can be systematically varied. Recent development in DNA research has produced not only sophisticated nanostructures but also new functions including ligand binding and catalysis. For noncationic liposomes, a DNA is typically covalently linked to a hydrophobic or lipid moiety that can be inserted into lipid membranes. In this article, we discuss fundamental biointerfaces formed between DNA and noncationic liposomes. The methods to prepare such conjugates and the interactions at the membrane interfaces are also discussed. The effect of DNA lateral diffusion on fluid bilayer membranes and the effect of membrane on DNA assembly are emphasized. DNA hybridization can be programmed to promote fusion of lipid membranes. Representative applications of this conjugate for drug delivery, biosensor development, and directed assembly of materials are briefly described toward the end. Some future research directions are also proposed to further understand this biointerface.

中文翻译：

---

DNA寡核苷酸功能化的脂质体：生物共轭化学，生物接口和应用程序。

DNA与脂质体的接口产生了各种各样的可编程软材料，设备和药物传递载体。通过简单地控制脂质体组成，可以系统地改变双层流动性，脂质结构域形成和表面电荷。DNA研究的最新进展不仅产生了复杂的纳米结构，而且还产生了包括配体结合和催化在内的新功能。对于非阳离子脂质体，DNA通常与可插入脂质膜的疏水或脂质部分共价连接。在本文中，我们讨论了DNA与非阳离子脂质体之间形成的基本生物界面。还讨论了制备此类缀合物的方法以及在膜界面的相互作用。强调了DNA横向扩散对流体双层膜的影响以及膜对DNA组装的影响。可以对DNA杂交进行编程，以促进脂质膜的融合。最后将简要介绍这种偶联物在药物递送，生物传感器开发和材料的定向组装中的代表性应用。还提出了一些未来的研究方向，以进一步了解这种生物界面。