Tryptophan (TRP) and dopamine (DA) pathways are of great importance for their related pathology and physiology. In the present study, a new reliable and sensitive analytical method was developed and validated for 12 neuroactive metabolites in TRP and DA pathways in mouse serum and brain by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). The method exhibited good sensitivity as the lower limit of detections ranged from 0.10 to 0.50 ng/ml and the lower limit of quantifications ranged from 0.20 to 2.00 ng/ml by derivatization with dansyl chloride (DNS-Cl) following solid phase extraction (SPE) on C18 cartridges. Good linearity (R² > 0.99), intra-day precision (<9.8% in serum and <8.8% in brain), inter-day precision (<8.9% in serum and <8.5% in brain) and accuracy (90.3%–110.3% in serum and 86.5%–114.0% in brain) were obtained. The method was successfully applied in measuring 12 neuroactive metabolites in TRP and DA pathways in serum and brain samples of male and female mice to explore the differences between genders. As a result, DA and the turnover of DA to 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxtryptamine (5-HT) to TRP and 5-hydroxyindole acetic acid (5-HIAA) to 5-HT in the serum and norepinephrine (NE) in the brain were significantly different between genders.

色氨酸（TRP）和多巴胺（DA）途径对其相关的病理学和生理学至关重要。在本研究中，开发了一种新的可靠而灵敏的分析方法，并通过超高效液相色谱-串联质谱（UHPLC-MS / MS）对小鼠血清和脑中TRP和DA途径中的12种神经活性代谢物进行了验证。 。固相萃取（SPE）后通过丹磺酰氯（DNS-Cl）衍生化，检测的下限为0.10至0.50 ng / ml，定量的下限为0.20至2.00 ng / ml，该方法显示出良好的灵敏度。在C18墨盒上。良好的线性度（R ² > 0.99），日内精确度（血清中<9.8％，大脑中<8.8％），日间精密度（血清中<8.9％，大脑中<8.5％）和准确性（血清中90.3％–110.3％在大脑中获得了86.5％–114.0％）。该方法已成功用于测定雄性和雌性小鼠血清和脑样本中TRP和DA通路中的12种神经活性代谢物，以探索性别之间的差异。结果，血清中的DA和DA转换为3,4-二羟基苯基乙酸（DOPAC），5-羟色胺（5-HT）转换为TRP和5-羟基吲哚乙酸（5-HIAA）转换为5-HT的周转率。大脑中的去甲肾上腺素（NE）在性别上有显着差异。