Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing,Angewandte Chemie International Edition

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Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-07-13 , DOI: 10.1002/anie.201806941
Wenhua Zhou ₁ , Haodong Cui ₁ , Liming Ying ₂ , Xue-Feng Yu ₁

Affiliation

A biodegradable two‐dimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3‐BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradation‐associated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3‐BPs thus provide efficient genome editing and gene silencing in vitro and in vivo at a relatively low dose as compared with other nanoparticle‐based delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications.

中文翻译：

黑色磷纳米片增强了CRISPR / Cas9的胞质传递和释放，用于基因组编辑

成功地建立了一个可生物降解的二维（2D）递送平台，该平台基于在C末端（Cas9N3）上加载了带有三个核定位信号（NLSs）的Cas9核糖核蛋白的黑磷纳米片（BPs）。Cas9N3-BPs通过膜渗透和内吞途径有效进入细胞，随后是BPs生物降解相关的内体逃逸和负载的Cas9N3复合物的胞质释放。因此，与其他基于纳米颗粒的递送平台相比，Cas9N3-BP在相对较低的剂量下即可在体内外进行有效的基因组编辑和基因沉默。这个可生物降解的2D传递平台为CRISPR / Cas9核糖核蛋白和其他生物活性生物活性大分子提供了一种通用的胞质传递方法。

更新日期：2018-07-13

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