Antibiotic Resistome Associated with Small-Scale Poultry Production in Rural Ecuador,Environmental Science & Technology

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Antibiotic Resistome Associated with Small-Scale Poultry Production in Rural Ecuador
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2018-07-10 , DOI: 10.1021/acs.est.8b01667
Xueping Guo _{1,

2,

3} , Robert D. Stedtfeld ₄ , Hayden Hedman ₅ , Joseph N. S. Eisenberg ₆ , Gabriel Trueba ₇ , Daqiang Yin ₁ , James M. Tiedje _{2,

3,

8} , Lixin Zhang _{8,

9}

Affiliation

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824, United States
Center for Microbial Ecology, East Lansing, Michigan 48824, United States
Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan 48824, United States
School for Environment and Sustainability, University of Michigan, Ann Arbor, Michigan 48109, United States
Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109, United States
Institute of Microbiology, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Quito 170157, Ecuador
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824, United States
Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, Michigan 48824, United States

Small-scale poultry farming is common in rural communities across the developing world. To examine the extent to which small-scale poultry farming serves as a reservoir for resistance determinants, the resistome of fecal samples was compared between production chickens that received antibiotics and free-ranging household chickens that received no antibiotics from a rural village in northern Ecuador. A qPCR array was used to quantify antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) using 248 primer pairs; and the microbiome structure was analyzed via 16S rRNA gene sequencing. A large number of ARGs (148) and MGEs (29) were detected. The ARG richness in production chickens was significantly higher than that of household chickens with an average of 15 more genes detected (p < 0.01). Moreover, ARGs and MGEs were much more abundant in production chickens than in household chickens (up to a 157-fold difference). Production chicken samples had significantly lower taxonomic diversity and were more abundant in Gammaproteobacteria, Betaproteobacteria, and Flavobacteria. The high abundance and diversity of ARGs and MGEs found in small-scale poultry farming was comparable to the levels previously found in large scale animal production, suggesting that these chickens could act as a local reservoir for spreading ARGs into rural communities.

中文翻译：

厄瓜多尔农村地区小规模家禽生产中的抗生素抗药性

在发展中世界的农村社区中，小规模的家禽养殖很普遍。为了检验小规模家禽养殖在多大程度上用作抵抗力决定因素的储藏库，比较了接受抗生素治疗的生产鸡与未接受厄瓜多尔北部农村地区抗生素治疗的散养家用鸡粪便样品的抵抗力。qPCR阵列用于使用248对引物对抗生素抗性基因（ARG）和移动遗传元件（MGE）进行定量；并通过16S rRNA基因测序对微生物组结构进行了分析。检测到大量的ARG（148）和MGE（29）。生产的鸡的ARG丰富度显着高于家用的鸡，平均检测出15个以上的基因（p<0.01）。此外，生产型鸡中的ARG和MGE比家用鸡中的丰富得多（相差157倍）。生产的鸡样品具有明显较低的分类学多样性，并且在γ-变形杆菌，β-变形杆菌和黄杆菌中含量更高。在小规模的家禽养殖中发现的ARG和MGE的高度丰富和多样性与以前在大规模动物生产中发现的水平相当，这表明这些鸡可以作为将ARG传播到农村社区的本地水库。

更新日期：2018-07-12

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