Structural Insights into the Regulation of Staphylococcus aureus Phosphofructokinase by Tetramer–Dimer Conversion,Biochemistry

当前位置： X-MOL 学术 › Biochemistry › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Structural Insights into the Regulation of Staphylococcus aureus Phosphofructokinase by Tetramer–Dimer Conversion
Biochemistry ( IF 2.9 ) Pub Date : 2018-06-25 00:00:00 , DOI: 10.1021/acs.biochem.8b00028
Tian Tian _{1,

2} , Chengliang Wang _{1,

2} , Minhao Wu _{1,

2} , Xuan Zhang _{1,

2} , Jianye Zang _{1,

2}

Affiliation

Most reported bacterial phosphofructokinases (Pfks) are tetramers that exhibit activity allosterically regulated via conformational changes between the R and T states. We report that the Pfk from Staphylococcus aureus NCTC 8325 (SaPfk) exists as both an active tetramer and an inactive dimer in solution. Multiple effectors, including pH, ADP, ATP, and adenylyl-imidodiphosphate (AMP-PNP), cause equilibrium shifts from the tetramer to dimer, whereas the substrate F6P stabilizes SaPfk tetrameric assembly. Crystal structures of SaPfk in complex with different ligands and biochemical analysis reveal that the flexibility of the Gly150-Leu151 motif in helix α7 plays a role in tetramer–dimer conversion. Thus, we propose a molecular mechanism for allosteric regulation of bacterial Pfk via conversion between the tetramer and dimer in addition to the well-characterized R-state/T-state mechanism.

中文翻译：

四聚体-二聚体转化对金黄色葡萄球菌磷酸果糖激酶调控的结构性见解

大多数报道的细菌磷酸果糖激酶（Pfks）是四聚体，其表现出通过R和T状态之间的构象变化而变构调节的活性。我们报告说，来自金黄色葡萄球菌NCTC 8325（Sa Pfk）的Pfk作为活性四聚体和非活性二聚体存在于溶液中。多种效应物，包括pH值，ADP，ATP和腺苷亚胺基二磷酸（AMP-PNP），会导致从四聚体到二聚体的平衡转移，而底物F6P使Sa Pfk四聚体组装稳定。Sa的晶体结构具有不同配体的Pfk和生化分析表明，螺旋α7中Gly150-Leu151基序的柔韧性在四聚体-二聚体转化中起作用。因此，我们提出了一种分子机制，通过表征四聚体和二聚体之间的转化，以及对R-state / T-state的充分表征，对细菌Pfk进行变构调节。

更新日期：2018-06-25

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11