Imaging of Lipids in Native Human Bone Sections Using TOF–Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap Mass Spectrometry, and Orbitrap–Secondary Ion Mass Spectrometry,Analytical Chemistry

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Imaging of Lipids in Native Human Bone Sections Using TOF–Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap Mass Spectrometry, and Orbitrap–Secondary Ion Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-06-26 00:00:00 , DOI: 10.1021/acs.analchem.8b00892
Kaija Schaepe , Dhaka R. Bhandari , Janina Werner ₁ , Anja Henss , Alexander Pirkl ₂ , Matthias Kleine-Boymann ₂ , Marcus Rohnke , Sabine Wenisch ₁ , Elena Neumann ₃ , Jürgen Janek , Bernhard Spengler

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A method is described for high-resolution label-free molecular imaging of human bone tissue. To preserve the lipid content and the heterogeneous structure of osseous tissue, 4 μm thick human bone sections were prepared via cryoembedding and tape-assisted cryosectioning, circumventing the application of organic solvents and a decalcification step. A protocol for comparative mass spectrometry imaging (MSI) on the same section was established for initial analysis with time-of-flight secondary ion mass spectrometry (TOF-SIMS) at a lateral resolution of 10 μm to <500 nm, followed by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) Orbitrap MSI at a lateral resolution of 10 μm. This procedure ultimately enabled MSI of lipids, providing the lateral localization of major lipid classes such as glycero-, glycerophospho-, and sphingolipids. Additionally, the applicability of the recently emerged Orbitrap–TOF-SIMS hybrid system was exemplarily examined and compared to the before-mentioned MSI methods.

中文翻译：

使用TOF-二次离子质谱，大气压扫描微探针基质辅助激光解吸/电离Orbitrap质谱和Orbitrap-二次离子质谱对自然人骨切片中的脂质成像

描述了一种用于人类骨组织的高分辨率无标记分子成像的方法。为了保留脂质含量和骨组织的异质结构，通过冷冻嵌入和胶带辅助冷冻切片制备了4μm厚的人骨切片，从而避免了有机溶剂的应用和脱钙步骤。建立了用于在同一部分进行比较质谱成像（MSI）的协议，用于使用飞行时间二次离子质谱（TOF-SIMS）在10μm至<500 nm的横向分辨率下进行初始分析，然后进行大气压以10μm的横向分辨率扫描微探针基质辅助激光解吸/电离（AP-SMALDI）Orbitrap MSI。此程序最终使脂质的MSI成为可能，从而提供了主要脂质类别（如甘油，甘油磷脂和鞘脂。另外，示例性地检查了最近出现的Orbitrap-TOF-SIMS混合系统的适用性，并将其与前面提到的MSI方法进行了比较。

更新日期：2018-06-26

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