The properties of DNA self-assembled monolayers (SAMs) have strong influences on the interfacial DNA–analyte binding behavior, which further affect the performance of biosensors built upon. In this work, we prepared binary thiolate DNA/6-ferrocenyl-1-hexanethiol (FcC6SH) SAMs on gold (DNA/FcC6S-Au) for convenient electrochemical characterization and subsequent data analysis. Our cyclic voltammetric (CV) studies confirmed that the redox responses of surface-tethered Fc and electrostatically bound [Ru(NH₃)₆]³⁺ are capable of providing quantitative information regarding the DNA film properties, including the surface density, structural heterogeneity, and molecular orientation under different preparation and measurement conditions. With the binary thiolate DNA/FcC6S-Au SAM prepared in the conventional post-assembly exchange protocol as a trial system, we are demonstrating the capability of introducing redox-active thiols as passivating and labeling reagents for preparing many other DNA-based biosensing interfaces via varied assembly steps and under different measurement conditions.

中文翻译：

---

黄金上的二硫代乙二酸酯DNA /二茂铁基自组装单层膜：探测生物传感界面的多功能平台。

DNA自组装单分子膜（SAM）的特性对界面DNA与分析物的结合行为有很大的影响，这进一步影响了建立在其上的生物传感器的性能。在这项工作中，我们在金（DNA / FcC6S-Au）上制备了二元硫醇盐DNA / 6-二茂铁基-1-己硫醇（FcC6SH）SAM，用于方便的电化学表征和后续数据分析。我们的循环伏安（CV）研究证实，表面束缚的Fc和静电结合的[Ru（NH ₃）₆ ] ³⁺的氧化还原反应能够提供有关DNA膜特性的定量信息，包括在不同制备和测量条件下的表面密度，结构异质性和分子取向。以常规的组装后交换协议中制备的二元硫醇盐DNA / FcC6S-Au SAM作为试验系统，我们证明了引入氧化还原活性硫醇作为钝化和标记试剂的能力，可通过以下方法制备许多其他基于DNA的生物传感界面不同的组装步骤和不同的测量条件。