Transformative Opportunities for Single-Cell Proteomics,Journal of Proteome Research

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Transformative Opportunities for Single-Cell Proteomics
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-07-19 , DOI: 10.1021/acs.jproteome.8b00257
Harrison Specht ₁ , Nikolai Slavov _{1,

2}

Affiliation

Many pressing medical challenges, such as diagnosing disease, enhancing directed stem-cell differentiation, and classifying cancers, have long been hindered by limitations in our ability to quantify proteins in single cells. Mass spectrometry (MS) is poised to transcend these limitations by developing powerful methods to routinely quantify thousands of proteins and proteoforms across many thousands of single cells. We outline specific technological developments and ideas that can increase the sensitivity and throughput of single-cell MS by orders of magnitude and usher in this new age. These advances will transform medicine and ultimately contribute to understanding biological systems on an entirely new level.

中文翻译：

单细胞蛋白质组学的变革机遇

许多紧迫的医学挑战，例如诊断疾病、增强干细胞定向分化和癌症分类，长期以来一直受到我们量化单细胞蛋白质能力的限制的阻碍。质谱 (MS) 有望通过开发强大的方法来对数千个单细胞中的数千种蛋白质和蛋白质形式进行常规定量，从而超越这些限制。我们概述了具体的技术发展和想法，可以将单细胞 MS 的灵敏度和通量提高几个数量级，并开创这个新时代。这些进步将改变医学，并最终有助于在全新的水平上理解生物系统。

更新日期：2018-07-20

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