Proteomic Analysis and Immuno-Profiling of Eastern India Russell’s Viper (Daboia russelii) Venom: Correlation between RVV Composition and Clinical Manifestations Post RV Bite,Journal of Proteome Research

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Proteomic Analysis and Immuno-Profiling of Eastern India Russell’s Viper (Daboia russelii) Venom: Correlation between RVV Composition and Clinical Manifestations Post RV Bite
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-07-10 , DOI: 10.1021/acs.jproteome.8b00291
Bhargab Kalita ₁ , Aparup Patra ₁ , Anjali Das ₁ , Ashis K Mukherjee ₁

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The proteomes of Russell’s viper venom (RVV) from Burdwan (RVV B) and Nadia (RVV N), the two districts of West Bengal, eastern India (EI), were investigated by gel-filtration chromatography (GFC) followed by tandem mass spectrometry of tryptic fragments of the fractions. A total of 73 and 69 proteins belonging to 15 snake venom protein families were identified in RVV B and RVV N, respectively, by MS/MS search against Viperidae (taxid 8689) protein entries of the nonredundant NCBI database. The minor differences in venom composition of both the EI RV were established unequivocally by their biochemical and pharmacological properties and by SDS-PAGE, gel filtration chromatography, and LC-MS/MS analyses. The composition of EI RVVs was well correlated with published reports on the pathophysiology of RV-envenomed patients from this part of the country. Venom–antivenom cross-reactivity determined by ELISA, Western blotting, and antivenomics approaches demonstrated poor recognition of low molecular mass (<20 kDa) RVV proteins by commercial polyvalent antivenoms, which was substantiated by neutralization of RVV enzymes by antivenom.

中文翻译：

印度东部罗素蝰蛇 (Daboia russselii) 毒液的蛋白质组学分析和免疫分析：RVV 组成与 RV 咬伤后临床表现之间的相关性

通过凝胶过滤色谱 (GFC) 和串联质谱法对来自印度东部 (EI) 西孟加拉邦两个地区 Burdwan (RVV B) 和 Nadia (RVV N) 的罗素蝰蛇毒液 (RVV) 的蛋白质组进行了研究级分的胰蛋白酶片段。通过对非冗余 NCBI 数据库的蝰蛇科 (taxid 8689) 蛋白质条目进行 MS/MS 搜索，分别在 RVV B 和 RVV N 中鉴定出属于 15 个蛇毒蛋白家族的总共 73 种和 69 种蛋白质。两种 EI RV 毒液成分的微小差异通过其生化和药理学特性以及 SDS-PAGE、凝胶过滤色谱和 LC-MS/MS 分析明确确定。 EI RVV 的组成与该地区已发表的 RV 感染患者的病理生理学报告密切相关。通过 ELISA、Western blotting 和抗蛇毒血清学方法测定的毒液-抗蛇毒血清交叉反应性表明，商业多价抗蛇毒血清对低分子量 (<20 kDa) RVV 蛋白的识别能力较差，这一点通过抗蛇毒血清中和 RVV 酶得到证实。

更新日期：2018-07-12

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