AbstractThe authors describe a new coating for use in solid-phase microextraction (SPME). Silver nanoparticles (AgNPs) were prepared by using gallic acid or glucose as the reducing agents, and then supported onto a stainless steel wire that was previously coated with a silver mirror. Coating with AgNPs was performed by a layer-by-layer approach of up to eight cycles of consecutive deposition of AgNPs and the thiol linker 1,8-octanedithiol. This procedure allows proper control of the coating thickness. Thicknesses are 3.2 μm and 3.5 μm with AgNPs obtained with gallic acid and glucose, respectively. This is in agreement with theoretical estimations (3.8 μm). The fibers were used in the direct-immersion SPME-GC-FID determination of 16 polycyclic aromatic hydrocarbons (PAHs) from different waters. The performance of the method was compared to the one using polydimethylsiloxane fibers (100 μm), which is the most suitable commercial SPME fiber for PAHs. Despite the low thickness of the AgNP coatings (compared to PDMS), the analytical features of the method using the most adequate coating (AgNPs prepared with gallic acid) include: (a) limits of detection down to 0.6 ng·mL−1; (b) intra-day, inter-day, and inter-fiber precisions (expressed as RSDs) lower than 22, 26 and 25%, respectively; and (c) an operational lifetime of ~150 extractions/desorption cycles. The analysis of various spiked environmental waters using these fibers resulted in adequate analytical performance. Graphical abstractSilver nanoparticle based coatings for solid-phase microextraction fibers were prepared by a layer-by-layer approach. They were used for determination of 16 PAHs in waters by gas chromatography. Limits of detection are < 14 μg·L−1 and intra-day, inter-day, and inter-fiber precisions are <26%.

中文翻译：

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用 GC-FID 测定多环芳烃之前，将银纳米颗粒负载在不锈钢线上用于直接浸入式固相微萃取

摘要作者描述了一种用于固相微萃取 (SPME) 的新型涂层。使用没食子酸或葡萄糖作为还原剂制备银纳米粒子 (AgNPs)，然后将其负载在预先涂有银镜的不锈钢线上。AgNPs 的涂层是通过最多八个循环的连续沉积 AgNPs 和硫醇接头 1,8-辛二硫醇的逐层方法进行的。该程序允许适当控制涂层厚度。用没食子酸和葡萄糖获得的 AgNP 的厚度分别为 3.2 μm 和 3.5 μm。这与理论估计值 (3.8 μm) 一致。这些纤维用于直接浸入式 SPME-GC-FID 测定来自不同水域的 16 种多环芳烃 (PAH)。将该方法的性能与使用聚二甲基硅氧烷纤维 (100 μm) 的方法进行比较，聚二甲基硅氧烷纤维是最适合多环芳烃的商业 SPME 纤维。尽管 AgNP 涂层的厚度较薄（与 PDMS 相比），但使用最合适涂层（用没食子酸制备的 AgNP）的方法的分析特征包括：(a) 检测限低至 0.6 ng·mL-1；(b) 日内、日间和纤维间精度（以 RSD 表示）分别低于 22%、26% 和 25%；(c) 使用寿命约为 150 次提取/解吸循环。使用这些纤维对各种加标环境水进行分析，获得了足够的分析性能。图形摘要用于固相微萃取纤维的银纳米粒子涂层是通过逐层方法制备的。它们用于通过气相色谱法测定水中的 16 种多环芳烃。检测限< 14 μg·L-1，日内、日间和纤维间精度<26%。