Dual-Responsive Self-Assembled Monolayer for Specific Capture and On-Demand Release of Live Cells,Langmuir

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Dual-Responsive Self-Assembled Monolayer for Specific Capture and On-Demand Release of Live Cells
Langmuir ( IF 3.7 ) Pub Date : 2018-06-22 00:00:00 , DOI: 10.1021/acs.langmuir.8b00676
Xia Gao _{1,

2} , Qiang Li ₁ , Fengchao Wang ₁ , Xuehui Liu ₁ , Dingbin Liu _{1,

2}

Affiliation

We report a dual-responsive self-assembled monolayer (SAM) on a well-defined rough gold substrate for dynamic capture and release of live cells. By incorporating 5′-triphosphate (ATP) aptamer into a SAM, we can accurately isolate specific cell types and subsequently release captured cells at either population or desired-group (or even single-cell) levels. On one hand, the whole SAMs can be disassembled through addition of ATP solution, leading to the entire release of the captured cells from the supported substrate. On the other hand, desired cells can be selectively released using near-infrared light irradiation, with relatively high spatial and temporal precision. The proposed dual-responsive cell capture-and-release system is biologically friendly and is reusable with another round of modification, showing great usefulness in cancer diagnosis and molecular analysis.

中文翻译：

双响应自组装单层膜，用于活细胞的特异性捕获和按需释放

我们报告了在明确定义的粗糙金基底上的双响应自组装单分子膜（SAM），用于动态捕获和释放活细胞。通过将5'-三磷酸（ATP）适体掺入SAM中，我们可以准确地分离特定的细胞类型，并随后以群体或所需组（甚至单细胞）水平释放捕获的细胞。一方面，可以通过添加ATP溶液来分解整个SAM，从而导致捕获的细胞从支持的底物中完全释放出来。另一方面，可以使用近红外光照射以相对较高的空间和时间精度选择性地释放期望的细胞。拟议中的双重反应性细胞捕获和释放系统具有生物学上的优势，可在另一轮修改后重复使用，

更新日期：2018-06-22

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