A method for simultaneous determination of water‐soluble vitamins B₁, B₂, B₃ (nicotinamide), B₅, B₆ (pyridoxamine), B₉ and fat‐soluble vitamins E (α‐tocopherol) and K₁ in tears, and B₁, B₂, B₃, B₅, B₆, B₉, A (retinol), and E in blood serum is described. Liquid chromatography‐mass spectrometry (LC‐MS) was used with a ternary mobile phase of water and acetonitrile containing 0.1% formic acid and methanol containing 5 mM ammonium formate. Vitamins were quantified using an internal standard method. Using 25 μL injection volumes, the limits of detection were in the range of 0.066‐5.3 ng in tear, and 0.087‐1.1 ng in serum with linear responses for all vitamins. Intra‐ and inter‐day precision and recoveries were satisfactory. This is the first study to demonstrate simultaneous vitamin detections in microliters of biological samples which has distinct advantages in many diagnostic applications with limited available fluids (e.g., tears; elderly anemic blood) or sampling small subjects (e.g., rodents). © 2018 American Institute of Chemical Engineers AIChE J, 64: 3709–3718, 2018

中文翻译：

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通过LC-MS对微升生物样品中的维生素进行检测和定量以用于临床筛查

一种同时测定眼泪中水溶性维生素B ₁，B ₂，B ₃（烟酰胺），B ₅，B ₆（吡rid胺），B ₉和脂溶性维生素E（α-生育酚）和K ₁的方法，和B ₁，B ₂，B ₃，B ₅，B ₆，B ₉描述了血清中的A，视黄醇和E。液相色谱-质谱（LC-MS）用于水和含0.1％甲酸的乙腈和含5 mM甲酸铵的甲醇的三元流动相。使用内标法对维生素进行定量。使用25μL进样量，检测限在泪液中为0.066-5.3 ng，在血清中为0.087-1.1 ng，所有维生素均具有线性响应。日间和日间精度和回收率令人满意。这是第一项证明同时检测微升生物样品中维生素的研究，这在许多诊断应用中具有有限的可用流体（例如眼泪；老年贫血）或对小对象（例如啮齿动物）进行采样的独特优势。©2018美国化学工程师学会AIChE J，64：3709–3718，2018