Rearranging the topology of an optimized genetically encoded Ca²⁺ indicator produces a variant that retains the favorable properties of the original but also has new termini in closer proximity to the chromophore. Fusion of a bright luciferase to the new N terminus results in a highly effective glow‐in‐the‐dark Ca²⁺ indicator.

中文翻译：

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基于GCaMP6s拓扑变异的生物发光Ca2 +指示剂

重新排列优化的遗传编码的Ca ²⁺指示剂的拓扑结构会产生一个变体，该变体既保留原始的有利特性，又在靠近生色团的位置具有新的末端。将明亮的荧光素酶融合到新的N末端可产生高效的黑暗中Ca ²⁺指示剂。