A fluorescent probe is constructed to quickly and accurately detect bisulfite. The detection mechanism was considered to operate via the bisulfite, causing deprotonation of amido bond of 8-aminoquinoline derivative leading to fluorescence enhancement. The probe has a good selectivity for bisulfite over the other competitive substances such as F⁻, Cl⁻, Br⁻, I⁻, C₂O₄²⁻, NO₂⁻, ClO₄⁻, OAc⁻, PO₄³⁻, CO₃²⁻, HCO₃⁻, HS⁻, HSO₄⁻, SCN⁻, SO₄²⁻, S₂O₃²⁻, Cys, Hcy and GSH. There is a good linear relationship between fluorescence intensity and the concentration of bisulfite in the range of 0–200 μM. The limit of detection was calculated to be 0.61 μM. Furthermore, the probe has also been successfully used for imaging bisulfite in living cells.

荧光探针可快速准确地检测亚硫酸氢盐。该检测机制被认为是通过亚硫酸氢盐起作用的，导致8-氨基喹啉衍生物的酰胺键去质子化，从而导致荧光增强。探头具有超过其他竞争物质例如F为亚硫酸氢盐以良好的选择性^- ，氯^- ，溴^- ，我^-，C ₂ Ò ₄ ^2-，NO ₂ ^-，CLO ₄ ^-，OAC ^-，PO ₄ ^3-， CO ₃ ²⁻，HCO ₃ ⁻，HS ⁻，HSO₄ ^-，SCN ^-，SO ₄ ^2-，S ₂ ö ₃ ^2-，半胱氨酸，同型半胱氨酸和谷胱甘肽。荧光强度与亚硫酸氢盐浓度在0-200μM之间具有良好的线性关系。计算的检出限为0.61μM。此外，该探针也已成功用于活细胞中亚硫酸氢盐的成像。