Subcellular Distribution and Chemical Forms of Pb in Corn: Strategies Underlying Tolerance in Pb Stress,Journal of Agricultural and Food Chemistry

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Subcellular Distribution and Chemical Forms of Pb in Corn: Strategies Underlying Tolerance in Pb Stress
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-22 00:00:00 , DOI: 10.1021/acs.jafc.7b03605
Jianling Sun ₁ , Liqiang Luo ₂

Affiliation

Studying the accumulation position and forms of heavy metals (HMs) in organisms and cells is helpful to understand the transport process and detoxification mechanism. As typical HMs, lead (Pb) subcellular content, localization, and speciation of corn subcellular fractions were studied by a series of technologies, including transmission electron microscopy, inductively coupled plasma mass spectrometry, and X-ray absorption near edge structure. The results revealed that the electrodense granules of Pb were localized in the cell wall, intercellular space, and plasma membranes. About 71% Pb was localized at the cell wall and soluble fraction. In cell walls, the total amount of pyromorphite and Pb carbonate was about 80% and the remaining was Pb stearate. In the nuclear and chloroplast fraction, which demonstrated significant changes, major speciations were Pb sulfide (72%), basic Pb carbonate (16%), and Pb stearate (12%). Pb is blocked by cell walls as pyromorphite and Pb carbonate sediments and compartmentalized by vacuoles, which both play an inportant role in cell detoxification. Besides, sulfur-containing compounds form inside the cells.

中文翻译：

玉米中铅的亚细胞分布和化学形式：铅胁迫耐受性的基本策略

研究有机物和细胞中重金属（HMs）的积累位置和形式有助于理解转运过程和排毒机理。作为典型的HM，通过一系列技术研究了玉米亚细胞部分中铅（Pb）的亚细胞含量，定位和形态，包括透射电子显微镜，电感耦合等离子体质谱法和近边缘结构的X射线吸收。结果表明，Pb的电极胶颗粒位于细胞壁，细胞间隙和质膜中。约71％的Pb位于细胞壁和可溶性级分中。在细胞壁中，亚硫铁矿和碳酸铅的总量约为80％，其余为硬脂酸铅。在显示出明显变化的核和叶绿体部分，主要形态为硫化铅（72％），碱式碳酸铅（16％）和硬脂酸铅（12％）。铅被作为硫铁矿和碳酸铅沉淀的细胞壁阻塞，并被液泡隔开，这两者在细胞排毒中都起着重要的作用。此外，在细胞内部形成含硫化合物。

更新日期：2018-06-22

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