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Nanostructured aptamer-based sensing platform for highly sensitive recognition of myoglobin
Microchimica Acta ( IF 5.3 ) Pub Date : 2018-06-21 , DOI: 10.1007/s00604-018-2860-0
Neda Ghafori Nia , Azadeh Azadbakht

AbstractA composite was prepared from PtSn nanoparticles and carbon nanotubes (PtSnNP/CNTs) and applied to the electrochemical determination of myoglobin (Mb). An Mb-aptamer was immobilized on a glassy carbon electrode (GCE), and hexcyanoferrate was used as an electrochemical probe. The PtSnNP/CNTs were synthesized by a microwave-aided ethylene glycol reduction method. Detection is based on electron transfer inhibition that is caused by the folding and conformational change of the Mb-aptamer in the presence of Mb. The amperometric signal for hexacyanoferrate, best measured at 0.2 V vs. Ag/AgCl depends on the concentration of Mb that interacts with the aptamer on the GCE. This approach is selective and sensitive for Mb due to (a) the highly specific recognition ability of the aptamer for Mb, (b) the powerful electronic properties of carbon nanotubes, (c) the arranged decoration of CNTs with PtSnNPs, and (d), the superior electron transfer to hexacyanoferrate. The assay is highly selective, with linear relationships from 0.01–1 nM and 10 nM–200 nM, and a limit of detection as low as 2.2 ± 0.1 pM. The modified GCE was applied to the quantitation of Mb in spiked human serum samples. Graphical abstractSchematic illustration of the method for Mb detection.

中文翻译:

基于纳米结构适体的传感平台用于肌红蛋白的高灵敏度识别

摘要 制备了 PtSn 纳米颗粒和碳纳米管 (PtSnNP/CNTs) 的复合材料,并应用于肌红蛋白 (Mb) 的电化学测定。将 Mb 适配体固定在玻璃碳电极 (GCE) 上,使用六氰基铁酸盐作为电化学探针。PtSnNP/CNTs 是通过微波辅助乙二醇还原法合成的。检测基于在 Mb 存在下由 Mb 适配体的折叠和构象变化引起的电子转移抑制。六氰基铁酸盐的安培信号,最好在 0.2 V vs. Ag/AgCl 下测量,取决于与 GCE 上的适体相互作用的 Mb 浓度。这种方法对 Mb 具有选择性和敏感性,因为 (a) 适配体对 Mb 的高度特异性识别能力,(b) 碳纳米管强大的电子特性,(c) 用 PtSnNPs 对 CNT 的排列装饰,以及 (d) 向六氰基铁酸盐的优异电子转移。该测定具有高度选择性,线性关系为 0.01–1 nM 和 10 nM–200 nM,检测限低至 2.2 ± 0.1 pM。改进的 GCE 用于定量人血清样品中的 Mb。图形摘要 Mb 检测方法的示意图。
更新日期:2018-06-21
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