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Directed Evolution of Protein Catalysts
Annual Review of Biochemistry ( IF 12.1 ) Pub Date : 2018-06-20 00:00:00 , DOI: 10.1146/annurev-biochem-062917-012034
Cathleen Zeymer 1 , Donald Hilvert 1
Affiliation  

Directed evolution is a powerful technique for generating tailor-made enzymes for a wide range of biocatalytic applications. Following the principles of natural evolution, iterative cycles of mutagenesis and screening or selection are applied to modify protein properties, enhance catalytic activities, or develop completely new protein catalysts for non-natural chemical transformations. This review briefly surveys the experimental methods used to generate genetic diversity and screen or select for improved enzyme variants. Emphasis is placed on a key challenge, namely how to generate novel catalytic activities that expand the scope of natural reactions. Two particularly effective strategies, exploiting catalytic promiscuity and rational design, are illustrated by representative examples of successfully evolved enzymes. Opportunities for extending these approaches to more complex biocatalytic systems are also considered.

中文翻译:


蛋白质催化剂的定向进化

定向进化是一种强大的技术,可为各种生物催化应用生成量身定制的酶。遵循自然进化的原理,诱变和筛选或选择的迭代循环可用于修饰蛋白质特性,增强催化活性或开发用于非天然化学转化的全新蛋白质催化剂。这篇综述简要地概述了用于产生遗传多样性并筛选或选择改良的酶变体的实验方法。重点放在一个关键挑战上,即如何产生新的催化活性,从而扩大自然反应的范围。成功开发的酶的代表性实例说明了利用催化混杂和合理设计的两种特别有效的策略。

更新日期:2018-06-20
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