当前位置: X-MOL 学术Annu. Rev. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Along the Central Dogma—Controlling Gene Expression with Small Molecules
Annual Review of Biochemistry ( IF 12.1 ) Pub Date : 2018-06-20 00:00:00 , DOI: 10.1146/annurev-biochem-060614-033923
Tilman Schneider-Poetsch 1 , Minoru Yoshida 1, 2
Affiliation  

The central dogma of molecular biology, that DNA is transcribed into RNA and RNA translated into protein, was coined in the early days of modern biology. Back in the 1950s and 1960s, bacterial genetics first opened the way toward understanding life as the genetically encoded interaction of macromolecules. As molecular biology progressed and our knowledge of gene control deepened, it became increasingly clear that expression relied on many more levels of regulation. In the process of dissecting mechanisms of gene expression, specific small-molecule inhibitors played an important role and became valuable tools of investigation. Small molecules offer significant advantages over genetic tools, as they allow inhibiting a process at any desired time point, whereas mutating or altering the gene of an important regulator would likely result in a dead organism. With the advent of modern sequencing technology, it has become possible to monitor global cellular effects of small-molecule treatment and thereby overcome the limitations of classical biochemistry, which usually looks at a biological system in isolation. This review focuses on several molecules, especially natural products, that have played an important role in dissecting gene expression and have opened up new fields of investigation as well as clinical venues for disease treatment.

中文翻译:


沿着中央教条—用小分子控制基因表达

在现代生物学的早期,人们就提出了分子生物学的中心教条,即DNA被转录为RNA,而RNA被翻译为蛋白质。早在1950年代和1960年代,细菌遗传学首次为理解生命作为大分子的遗传编码相互作用开辟了道路。随着分子生物学的进步和我们对基因控制的了解的加深,越来越明显的是表达依赖于更多水平的调控。在解剖基因表达机制的过程中,特定的小分子抑制剂发挥了重要作用,并成为有价值的研究工具。小分子比遗传工具具有明显的优势,因为它们可以在任何所需的时间点抑制过程,而突变或改变重要调控因子的基因很可能会导致死亡的生物体。随着现代测序技术的出现,监测小分子处理的整体细胞效应成为可能,从而克服了传统生物化学的局限性,而传统生物化学通常只能孤立地看待生物系统。这篇综述着重于几种分子,尤其是天然产物,这些分子在解剖基因表达中发挥了重要作用,并开辟了新的研究领域以及疾病治疗的临床场所。

更新日期:2018-06-20
down
wechat
bug