Trace minerals are vital in the antioxidant response during oxidative stress; however, limited research is available on the effects of trace mineral supplementation during an aflatoxin (AF) challenge. The objective of the study was to determine the effects of 2 subcutaneous injections of 15 mg/mL of Cu, 5 mg/mL of Se, 60 mg/mL of Zn, and 10 mg/mL of Mn (Multimin 90, Multimin North America, Fort Collins, CO) given at 1 mL/90.7 kg of average body weight in response to an AF challenge. Fifty-eight Holstein cows [body weight (mean ± SD) = 734 ± 6 0kg; days in milk = 191 ± 93] were assigned to 1 of 3 treatments in a randomized complete block design. The experimental period (63 d) was divided into an adaptation phase (d 1–56) and a measurement phase (d 57–63). From d 57 to 59, cows received an AF challenge that consisted of 100 μg of aflatoxin B₁/kg of dietary dry matter intake (DMI) administered orally via balling gun. Treatments were saline injection and no AF challenge (NEG), saline injection and AF challenge (POS), and trace mineral injection and AF challenge (MM). Injections were performed subcutaneously on d 1 and 29. Milk was sampled 3 times daily from d 56 to 63, blood was sampled on d 0, 56, 60, and 63, and liver samples were taken on d 0 and 60. Two treatment orthogonal contrasts [CONT1 (NEG vs. POS) and CONT2 (POS vs. MM)] were made. Cows in NEG had lower AF excretion in milk and greater 3.5% fat-corrected milk (32.1 ± 1.37 kg/d) compared with cows in POS (28.6 ± 1.43 kg/d). Feed efficiencies (3.5% fat-corrected milk/DMI, energy-corrected milk/DMI, and milk/DMI) were greater for cows in NEG (1.42 ± 0.07, 1.46 ± 0.07, and 1.45 ± 0.07, respectively) than cows in POS (1.16 ± 0.08, 1.18 ± 0.08, and 1.22 ± 0.07, respectively). Cows in POS had greater milk urea nitrogen and blood urea nitrogen than cows in MM. Liver concentrations of Se and Fe were greater for cows in MM compared with cows in POS. Cows in MM tended to have greater plasma glutathione peroxidase activity compared with cows in POS. An upregulation of liver GPX1 was observed for cows in POS compared with cows in MM. In conclusion, subcutaneous injection of trace minerals maintained an adequate antioxidant response when an AF challenge was present.

微量矿物质对于氧化应激中的抗氧化反应至关重要。但是，关于黄曲霉毒素（AF）攻击过程中微量矿物质补充的影响的研究还很有限。该研究的目的是确定两次皮下注射分别为15 mg / mL的Cu，5 mg / mL的Se，60 mg / mL的Zn和10 mg / mL的Mn的效果（Multimin 90，Multimin North America （美国佛罗里达州柯林斯堡）的平均体重为1 mL / 90.7 kg，以应对房颤。58头荷斯坦奶牛[体重（平均±SD）= 734±6 0公斤；在随机完整区组设计中，将牛奶中的天数= 191±93]分配给3种治疗方法之一。实验阶段（63 d）分为适应阶段（​​d 1–56）和测量阶段（d 57–63）。从d 57到59，母牛接受了一次AF挑战，其中包括100μg黄曲霉毒素B_1个/ kg通过球形喷枪口服的饮食干物质摄入量（DMI）。治疗方法为注射生理盐水和无房颤（NEG），注射生理盐水和房颤（POS）以及微量矿物质注射和房颤（MM）。在第1天和第29天皮下注射。从第56天到63天每天采样3次牛奶，在第0天，56天，60天和63天采样血液，在第0天和60天采样肝脏。对比[CONT1（NEG与POS）和CONT2（POS与MM）]。与POS奶牛（28.6±1.43 kg / d）相比，NEG奶牛的牛奶AF排泄量较低，经脂肪校正的牛奶（32.1±1.37 kg / d）较高。NEG母牛的饲料效率（3.5％脂肪校正的牛奶/ DMI，能量校正的牛奶/ DMI和牛奶/ DMI）要比POS母牛高（分别为1.42±0.07、1.46±0.07和1.45±0.07）。 （1.16±0.08，分别为1.18±0.08和1.22±0.07）。POS中的母牛比MM中的母牛具有更高的牛奶尿素氮和血液尿素氮。与POS奶牛相比，MM奶牛的肝脏中Se和Fe的浓度更高。与POS奶牛相比，MM奶牛倾向于具有更高的血浆谷胱甘肽过氧化物酶活性。肝脏上调GPX1观察到在POS奶牛在奶牛的MM比较。总之，当出现房颤时，皮下注射微量矿物质可保持足够的抗氧化反应。