Abstract Lignocellulosic single cell oil (SCO) is profound and promising in response to the increasing concerns on sustainable energy supply and environmental protection. Different bioprocesses from sodium hydroxide pretreated rice straw (SHPRS) to SCO were compared and the bioprocess c using the three-stage enzymatic hydrolysis was found to be the most efficient. It had the lowest enzyme input 153.1 FPIU cellulase/g SCO and the shortest time 222 h, but produced 42.0 g dry cell biomass and 23.4 g SCO from 342.0 g dry SHPRS. It had the highest lipid content 55.7%, and its productivities and yields were the highest. This study verified that on-site cellulase production by the mixed culture of Trichoderma reesei and Aspergillus niger and the three-stage enzymatic hydrolysis have the application value in SCO production from lignocelluloses by Mortierella isabellina.

中文翻译：

---

在现场纤维素酶生产的背景下，通过三阶段酶解降解氢氧化钠预处理稻草，伊莎贝氏被孢霉生产微生物油

摘要 木质纤维素单细胞油（SCO）在应对可持续能源供应和环境保护日益受到关注的情况下具有深远的意义和前景。比较了从氢氧化钠预处理稻草 (SHPRS) 到 SCO 的不同生物过程，发现使用三级酶水解的生物过程 c 是最有效的。它的酶输入最低为 153.1 FPIU 纤维素酶/g SCO，最短时间为 222 小时，但从 342.0 g 干 SHPRS 产生 42.0 g 干细胞生物量和 23.4 g SCO。其脂质含量最高，为55.7%，产量和产量最高。