A deoxynivalenol (DON) epitope clone (D₈) was obtained by phage display technology using anti-DON monoclonal antibodies as a target molecule. Subsequently, a DON antigen mimic (D₈-maltose-binding protein [MBP]) was synthesized by fusing the mimic epitope peptide with MBP. An enzyme-linked immunosorbent assay (ELISA) and urchin-like gold nanoparticle immunochromatographic assay was developed based on D₈-MBP for detection of DON in maize and wheat. The half-maximal inhibitory concentration, lower detection limit, and linear range of the D₈-MBP ELISA were 57.98 ± 0.97, 9.83, and 11.32–286.77 ng/mL, respectively. The sensitivity of the D₈-MBP ELISA was nearly 2.5 times higher than that of traditional ELISA using DON-bovine serum albumin (BSA). The detection threshold of the colloidal gold immunochromatographic assay for D₈-MBP was 25 ng/mL. Thus, D₈-MBP could be used to replace the traditional DON-BSA antigen for the immunological detection of DON, permitting low cost, rapid detection of DON.

使用抗DON单克隆抗体作为靶分子，通过噬菌体展示技术获得了脱氧雪腐烯（DON）表位克隆（D ₈）。随后，通过将模拟表位肽与MBP融合来合成DON抗原模拟物（D _8-麦芽糖结合蛋白[MBP]）。基于D ₈ -MBP，开发了酶联免疫吸附试验（ELISA）和海胆样金纳米颗粒免疫色谱分析方法，用于检测玉米和小麦中的DON。D ₈ -MBP ELISA的最大半数抑制浓度，下限和线性范围分别为57.98±0.97、9.83和11.32–286.77 ng / mL。D ₈的灵敏度-MBP ELISA是使用DON-牛血清白蛋白（BSA）的传统ELISA的近2.5倍。D ₈ -MBP的胶体金免疫色谱分析的检测阈值为25 ng / mL。因此，D ₈ -MBP可以用来代替传统的DON-BSA抗原进行DON的免疫学检测，从而可以低成本，快速地检测DON。