With the increasing demand for direct human and animal consumption seaweed farming is rapidly expanding worldwide. Macroalgae have colonized aquatic environments in which they are submitted to frequent changes in biotic and abiotic factors that can trigger oxidative stress (OS). Considering that isoprostanoid derivatives may constitute the most relevant OS biomarkers, we were interested to establish their profile in two red and four brown macroalgae. Seven phytoprostanes, three phytofuranes, and four isoprostanes were quantified through a new micro-LC-MS/MS method. The isoprostanoid contents vary greatly among all the samples, the ent-16(RS)-9-epi-ST-Δ¹⁴-10-PhytoF and the sum of 5-F_2t-IsoP and 5-epi-5F_2t-IsoP being the major compounds for most of the macroalgae studied. We further quantified these isoprostanoids in macroalgae submitted to heavy metal (copper) exposure. In most of the cases, their concentrations increased after 24 h of copper stress corroborating the original hypothesis. One exception is the decrease of ent-9-L1-PhytoP content in L. digitata.

随着人类和动物直接消费需求的增长，海藻养殖在全球范围内迅速发展。大型藻类已经定居在水生环境中，在这些环境中，它们经常遭受可能触发氧化应激（OS）的生物和非生物因子的频繁变化。考虑到异前列腺素衍生物可能构成最相关的OS生物标志物，我们有兴趣在两个红色和四个棕色大型藻类中建立它们的特征。通过一种新型的微LC-MS / MS方法对7种植物前列腺素，3种植物呋喃和4种异前列腺素进行了定量。所述isoprostanoid内容全部的样品中差别很大，ENT -16（RS）-9-外延-ST-Δ ¹⁴ -10- PhytoF和5-F的总和_2吨-IsoP和5-epi -5F _2t -IsoP是大多数研究的大型藻类的主要化合物。我们进一步量化了暴露于重金属（铜）的大型藻类中的这些异前列腺素类物质。在大多数情况下，铜胁迫24小时后它们的浓度增加，证实了最初的假设。一个例外是减少耳鼻喉科-9-L1-PhytoP内容属掌状。