Enhancing hydrophilicity of photoacoustic probes for effective ratiometric imaging of hydrogen peroxide†,Journal of Materials Chemistry B

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Enhancing hydrophilicity of photoacoustic probes for effective ratiometric imaging of hydrogen peroxide†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-06-22 00:00:00 , DOI: 10.1039/c8tb01158c
Xiaomei Lu _{1,

2,

3,

4,

5} , Meng Zhao _{1,

2,

3,

4,

5} , Pengfei Chen _{1,

2,

3,

4,

5} , Quli Fan _{2,

6,

7,

8,

9} , Wenjun Wang _{5,

10,

11,

12} , Wei Huang _{5,

13,

14,

15}

Affiliation

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM)
Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)
Nanjing Tech University (NanjingTech)
Nanjing 211816
China
Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors
Institute of Advanced Materials (IAM)
Nanjing University of Posts & Telecommunications
Nanjing 210023
Key Lab of Optical Communication Science and Technology of Shandong Province & School of Physics Science and Information Engineering
Liaocheng University
Liaocheng 252059
Shaanxi Institute of Flexible Electronics (SIFE)
Northwestern Polytechnical University (NPU)
Xi'an 710072

Hydrogen peroxide (H₂O₂) plays a significant role in regulating the redox balance in the living body. Compared with traditional imaging techniques, ratiometric photoacoustic imaging is no doubt a superior choice for H₂O₂ visualization. However, the difficult design of ratiometric probes with only one activatable agent that exhibits two changeable absorption peaks under H₂O₂ activation remains a big challenge. In this work, we developed a near-infrared absorbing probe, which responded to H₂O₂ selectively and permitted the ratiometric photoacoustic imaging of H₂O₂ in living mice. The probe was constructed from an Aza-BODIPY backbone attached with a benzeneboronic acid pinacol ester moiety though a quaternization reaction. Oligo(ethylene glycol) (OEG) was introduced into hydrophobic Aza-BODIPY to enhance the water-solubility of the probe. The OEG-Aza-BODIPY-BAPE probe exhibited sensitive and specific ratiometric PA signals towards H₂O₂. In vivo experiments also showed that the OEG-Aza-BODIPY-BAPE probe can be used for ratiometric PA imaging. Overall, our work illustrated a high sensitivity hydrophilic photoacoustic probe for ratiometric imaging of hydrogen peroxide in vitro and vivo.

中文翻译：

增强光声探针的亲水性，以便对过氧化氢进行有效的比例成像†

过氧化氢（H ₂ O ₂）在调节生物体内的氧化还原平衡中起着重要作用。与传统的成像技术相比，比例光声成像无疑是H ₂ O ₂可视化的最佳选择。然而，仅具有一种在H ₂ O ₂活化下表现出两个可变吸收峰的可活化剂的比率式探针的困难设计仍然是一个很大的挑战。在这项工作中，我们开发了一种近红外吸收探针，其回应至H ₂ ö ₂选择性地允许和H的比率的光声成像₂ ö ₂在活着的老鼠身上。通过季铵化反应，由连接有苯硼酸频哪醇酯部分的Aza-BODIPY主链构建探针。将寡聚乙二醇（OEG）引入疏水性Aza-BODIPY中以增强探针的水溶性。OEG-Aza-BODIPY-BAPE探针对H ₂ O ₂表现出灵敏且特定的比例PA信号。体内实验还表明，OEG-Aza-BODIPY-BAPE探针可用于比例式PA成像。总的来说，我们的工作展示了一种高灵敏度的亲水性光声探针，用于在体外和体内对过氧化氢进行比例成像。

更新日期：2018-06-22

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