A new nanosensor for the chiral recognition of cysteine enantiomers based on gold nanorods†,New Journal of Chemistry

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A new nanosensor for the chiral recognition of cysteine enantiomers based on gold nanorods†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-06-21 00:00:00 , DOI: 10.1039/c8nj02456a
Ruijun Li _{1,

2,

3,

4} , Changlong Zhu _{1,

2,

3,

4} , Wang Li _{1,

2,

3,

4} , Xintong Zhang _{1,

2,

3,

4} , Yibing Ji _{1,

2,

3,

4,

5}

Affiliation

An easy-to-use, fast and highly selective method, which used unmodified gold nanorods (GNRs) as a chiral nanosensor for the chiral recognition of cysteine (Cys) enantiomers, has been developed in this work. Under the optimized conditions, L-Cys caused GNRs aggregation, and the absorbance decrease of GNRs at a wavelength of 650 nm was 0.601. However, the GNRs were almost monodispersed and the absorbance decrease was only 0.289 for D-Cys. The combined assay process with the optical rotation of GNRs, and the chiral recognition phenomenon could be assigned to the fact that L-Cys induced the end-to-end assembly of GNRs through crosslinking agent Cu²⁺ ions. The detection limit of this method for L-Cys was 0.325 μM. The relative standard deviation (RSD) was 1.5% for trace L-Cys, which indicated that the method presented good precision for the analysis of trace L-Cys in solution samples. Futhermore, we demonstrated that the nanosensor possessed high selectivity for the chiral recognition of Cys enantiomers. The response of GNRs (ΔA_LD) to Cys enantiomers was 7.8 times to tryptophan (Try) enantiomers, and 9.2 times to phenylalanine (Phe) enantiomers. In our perception, the assay showed new opportunities for efficient enantiosensing strategies.

中文翻译：

基于金纳米棒的用于手性识别半胱氨酸对映体的新型纳米传感器†

这项工作开发了一种易于使用，快速且高度选择性的方法，该方法使用未修饰的金纳米棒（GNR）作为手性纳米传感器，用于手性识别半胱氨酸（Cys）对映体。在最佳条件下，L -Cys引起GNRs聚集，在650 nm波长处GNRs的吸光度降低为0.601。但是，GNR几乎是单分散的，D -Cys的吸光度降低仅为0.289 。结合了GNRs旋光性和手性识别现象的分析过程可以归因于L -Cys通过交联剂Cu ²⁺离子诱导GNRs端对端组装的事实。该方法对L的检测极限-Cys为0.325μM。痕量L -Cys的相对标准偏差（RSD）为1.5％，这表明该方法为溶液样品中痕量L -Cys的分析提供了很好的精度。此外，我们证明了该纳米传感器具有对Cys对映体的手性识别的高选择性。的GNR的响应（Δ甲_LD到半胱氨酸的对映体）为7.8倍为色氨酸（TRY）对映体和9.2倍至苯丙氨酸（Phe）对映体。在我们看来，该测定法显示了有效的增强策略的新机会。

更新日期：2018-06-21

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