Soil salinity is one of the major limitations that affects both plant and its soil environment, leading to reduced agricultural production. Evaluation of stress severity by plant physical and biochemical characteristics is an established way to study plant-salt stress interaction, but the halotolerant properties of plant growth promoting bacteria (PGPB) along with plant growth promotion is less studied till date. The aim of the present study was to elucidate the strategy, used by ACC deaminase-containing halotolerant Brevibacterium linens RS16 to confer salt stress tolerance in moderately salt-tolerant (FL478) and salt-sensitive (IR29) rice (Oryza sativa L.) cultivars. The plants were exposed to salt stress using 0, 50, and 100 mM of NaCl with and without bacteria. Plant physiological and biochemical characteristics were estimated after 1, 5, 10 days of stress application. H⁺ ATPase activity and the presence of hydroxyectoine gene (ectD) that is responsible for compatible solute accumulation were also analyzed in bacteria. The height and dry mass of bacteria inoculated plants significantly increased compared to salt-stressed plants, and the differences increased in time dependent manner. Bacteria priming reduced the plant antioxidant enzyme activity, lipid peroxidation and it also regulated the salt accumulation by modulating vacuolar H⁺ ATPase activity. ATPase activity and presence of hydroxyectoine gene in RS16 might have played a vital role in providing salt tolerance in bacteria inoculated rice cultivars. We conclude that dual benefits provided by the halotolerant plant growth promoting bacteria (PGPB) can provide a major way to improve rice yields in saline soil.

土壤盐分是影响植物及其土壤环境的主要限制之一，导致农业减产。通过植物物理和生化特性评估胁迫严重程度是研究植物盐胁迫相互作用的一种确定的方法，但是到目前为止，对促进植物生长的细菌（PGPB）和促进植物生长的耐盐特性的研究还很少。本研究的目的是阐明该策略，该策略被含ACC脱氨酶的卤代短杆菌细菌RS16用于赋予中等耐盐性（FL478）和盐敏感性（IR29）水稻（Oryza sativa）L.）品种。使用有，无细菌的0、50和100 mM NaCl将植物暴露于盐胁迫下。在施加胁迫1、5、10天后，估计植物的生理和生化特性。还分析了细菌中的H ⁺ ATPase活性和负责相容性溶质积累的羟基ectoine基因（ectD）的存在。与盐胁迫的植物相比，接种细菌的植物的高度和干重显着增加，并且差异随时间的变化而增加。细菌引发降低了植物抗氧化酶的活性，脂质过氧化作用，并且还通过调节液泡中的H ^+来调节盐的积累。ATPase活性。RS16中的ATPase活性和羟基连接素基因的存在可能在细菌接种的水稻品种中提供耐盐性中起着至关重要的作用。我们得出的结论是，卤虫植物生长促进细菌（PGPB）提供的双重好处可以提供一种提高盐渍土壤中水稻产量的主要方法。