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Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants
Food Research International ( IF 7.0 ) Pub Date : 2018-06-21 , DOI: 10.1016/j.foodres.2018.06.041
Ye Tian , Jaana Liimatainen , Anna Puganen , Hanna-Leena Alakomi , Jari Sinkkonen , Baoru Yang

In order to assist developing a natural, safe food-preservative, aqueous ethanolic extracts of leaves and berries of eight Finnish berry plants were fractionated with Sephadex LH-20 column chromatography. For each fraction, phenolic compounds were analyzed with NMR, UPLC-DAD-ESI-MS and HPLC-DAD. The antioxidant activities of the fractions were investigated using oxygen radical absorbance capacity (ORAC) assay, and the antibacterial activities were evaluated against foodborne pathogens Staphylococcus aureus and Escherichia coli. Antioxidant activities of the fractions correlated highly with both the total concentration and structural feature of phenolic compounds, including both flavonoids and non-flavonoid phenolics. ORAC value correlated strongly with the concentration of (+)-catechin, (−)-epicatecin, quercetin glycosides, and anthocyanins. Increase in size and number of sugar moieties may reduce the antioxidative activities of quercetin glycosides. Type of sugar moieties may have a significant role in influencing peroxyl-radicals scavenging ability of quercetin glycosides with monosaccharides as a single sugar moieties. Most of the fractions inhibited the target microbes. S. aureus strains expressed a higher sensitivity to phenolic compounds than E. coli strains.



中文翻译:

芬兰浆果植物提取物中的Sephadex LH-20分馏和酚类化合物的生物活性

为了帮助开发天然,安全的食品防腐剂,使用Sephadex LH-20柱色谱法对八种芬兰浆果植物的叶子和浆果的含水乙醇提取物进行了分馏。对于每个馏分,酚类化合物均通过NMR,UPLC-DAD-ESI-MS和HPLC-DAD分析。使用氧自由基吸收能力(ORAC)分析法研究了级分的抗氧化活性,并评估了对食源性病原体金黄色葡萄球菌大肠杆菌的抗菌活性。。馏分的抗氧化活性与包括黄酮类和非类黄酮酚类在内的酚类化合物的总浓度和结构特征高度相关。ORAC值与(+)-儿茶素,(-)-表苦素,槲皮素糖苷和花色苷的浓度密切相关。糖部分的大小和数量增加可能会降低槲皮素糖苷的抗氧化活性。糖部分的类型在影响槲皮素糖苷的过氧自由基清除能力方面具有重要作用,单糖作为单个糖部分。大多数馏分均抑制了目标微生物。与大肠杆菌菌株相比,金黄色葡萄球菌菌株对酚类化合物的敏感性更高。

更新日期:2018-06-21
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