The aim of the study was to determine the physiological indices of laboratory rats as a response to a diet consisting of a bread supplemented with freeze-dried water extracts of raw and roasted cocoa beans of Forastero variety. The influence of different cocoa extracts on oxidative stress, hyperglycemia, and lipid metabolism was studied during long-term feeding of laboratory rats with bread supplemented with cocoa extracts. During the 4-week experimental feeding samples of rats feces were subjected to analysis and after its completion, biological samples were collected i.e. intestines content, blood, and organs retrieved individually from each rat. The observed changes indicate the biological activity of polyphenol extracts and other components of cocoa beans present in the prepared extracts. The differences in the results obtained for the analyzed parameters of the gastrointestinal tract revealed that the cocoa bean extracts being the subject of this investigation differently affected the physicochemical properties of rats' intestines, including intestinal microflora. The results show the influence of the diet on the activity of both fecal and cecal enzymes. Applied nutrition treatment varied the concentration and total production of volatile fatty acids in the cecum. Furthermore, the diverse composition of the applied diet affected the level of some biochemical indicators of blood serum of rats. Additionally, to a small extent influenced the analyzed indicators of the antioxidative status of rats as characterized by the concentration of substances reacting with thiobarbituric acid in internal organs.

该研究的目的是确定实验大鼠的生理指标，以作为对饮食的反应，该饮食由面包和福斯特罗（Forastero）品种的生和烤可可豆的冻干水提取物组成。在长期喂食添加了可可提取物的面包的实验​​大鼠中，研究了不同可可提取物对氧化应激，高血糖和脂质代谢的影响。在为期4周的大鼠实验饲喂样品中，对粪便进行分析，并在完成后收集生物学样品，即分别从每只大鼠中回收的肠内容物，血液和器官。观察到的变化表明所制备的提取物中存在多酚提取物和可可豆其他成分的生物活性。对于胃肠道分析参数所获得的结果差异表明，可可豆提取物作为本研究的对象，对大鼠肠道（包括肠道菌群）的理化特性有不同的影响。结果表明饮食对粪便和盲肠酶活性的影响。应用的营养治疗改变了盲肠中挥发性脂肪酸的浓度和总产量。此外，所用饮食的不同组成影响了大鼠血清某些生化指标的水平。另外，在一定程度上影响了大鼠抗氧化状态的分析指标，其特征是与体内器官中与硫代巴比妥酸反应的物质的浓度有关。