Pancreatic α-amylase plays an important role in dietary starch hydrolysis in the small intestine and participates in enhanced glucose concentration after meals. It seems to be a problem for diabetic patients, who suffer from longer postprandial hyperglycemia after meal consumption than healthy people. There are commercially available drugs that inhibit α-amylase and thus reduce the postprandial hyperglycemia effect. However, these drugs may cause severe side effects. Conversely, some naturally occurring flavonoids were suggested to have an α-amylase-inhibiting effect without any side effects. There had been no rapid, undemanding method in terms of sample and reagent preparation that would enable screening of many potential inhibitors. Therefore, we developed an online capillary electrophoresis method to monitor α-amylase activity in the presence of an inhibitor. Each reaction constituent was introduced separately, directly into a capillary where the reagents were mixed by diffusion, which resulted in a 5-min analysis including conditioning of the capillary. We applied the method to test the inhibitory effect of flavonoid standards and their mixture and we investigated the inhibitory effect of ethanolic extract from Betula pendula bark. The developed method presents a faster and less expensive alternative to previously described offline methods.

中文翻译：

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毛细管电泳在线筛选α-淀粉酶抑制剂

胰腺α-淀粉酶在膳食中小肠淀粉的水解中起重要作用，并在饭后参与提高葡萄糖浓度。对于糖尿病患者而言，这似乎是一个问题，他们与健康人相比，在进餐后遭受餐后高血糖的时间更长。有可抑制α-淀粉酶并因此降低餐后高血糖作用的市售药物。但是，这些药物可能会导致严重的副作用。相反，某些天然存在的类黄酮被认为具有α-淀粉酶抑制作用，而没有任何副作用。在样品和试剂制备方面，还没有一种快速，不需要的方法可以筛查许多潜在的抑制剂。所以，我们开发了一种在线毛细管电泳方法来监测抑制剂存在下的α-淀粉酶活性。将每种反应成分分别直接引入毛细管中，在其中通过扩散将试剂混合，这导致5分钟的分析，包括对毛细管的调节。我们应用该方法测试了类黄酮标准品及其混合物的抑制作用，并研究了乙醇提取物的抑制作用。西南桦树皮。所开发的方法为先前描述的离线方法提供了一种更快，更便宜的替代方法。