During the last decades, the quality of aquatic ecosystems has been threatened by increasing levels of pollutions, caused by the discharge of man-made chemicals, both via accidental release of pollutants as well as a consequence of the constant outflow of inadequately treated wastewater effluents. For this reason, the European Union is updating its legislations with the aim of limiting the release of emerging contaminants. The Commission Implementing Decision (EU) 2015/495 published in March 2015 drafts a “Watch list” of compounds to be monitored Europe-wide. In this study, a methodology based on online solid-phase extraction (SPE) ultra-high-performance liquid chromatography coupled to a triple-quadrupole mass spectrometer (UHPLC-MS/MS) was developed for the simultaneous determination of the 17 compounds listed therein. The proposed method offers advantages over already available methods, such as versatility (all 17 compounds can be analyzed simultaneously), shorter time required for analysis, robustness, and sensitivity. The employment of online sample preparation minimized sample manipulation and reduced dramatically the sample volume needed and time required, dramatically the sample volume needed and time required, thus making the analysis fast and reliable. The method was successfully validated in surface water and influent and effluent wastewater. Limits of detection ranged from sub- to low-nanogram per liter levels, in compliance with the EU limits, with the only exception of EE2.

中文翻译：

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开发在线SPE-UHPLC-MS / MS方法以对欧盟中17种化合物进行多残留分析“观察名单”

在过去的几十年中，由于化学物质的排放，污染物的意外释放以及未经充分处理的废水不断不断流出所造成的污染水平的提高，水生生态系统的质量受到了威胁。因此，欧洲联盟正在更新其立法，以限制新兴污染物的释放。2015年3月发布的欧盟委员会执行决定（EU）2015/495拟定了在欧洲范围内要监测的化合物的“观察名单”。在这项研究中，开发了一种基于在线固相萃取（SPE）超高效液相色谱结合三重四极杆质谱仪（UHPLC-MS / MS）的方法，用于同时测定其中列出的17种化合物。与现有方法相比，所提出的方法具有很多优势，例如多功能性（可以同时分析所有17种化合物），分析所需的时间较短，鲁棒性和灵敏度高。在线样品制备的使用最大程度地减少了样品操作，并大大减少了所需的样品量和所需的时间，大大减少了所需的样品量和所需的时间，从而使分析快速可靠。该方法已在地表水以及进水和出水废水中得到了成功验证。除了EE2的检测限值外，检测限值的范围从每升亚纳克到低至每纳克低至最低。在线样品制备的使用最大程度地减少了样品操作，并大大减少了所需的样品量和所需的时间，大大减少了所需的样品量和所需的时间，从而使分析快速可靠。该方法已在地表水以及进水和出水废水中得到了成功验证。除了EE2的检测限值外，检测限值从每升亚纳克到低纳克不等。在线样品制备的使用最大程度地减少了样品操作，并大大减少了所需的样品量和所需的时间，大大减少了所需的样品量和所需的时间，从而使分析快速可靠。该方法已在地表水以及进水和出水废水中得到了成功验证。除了EE2的检测限值外，检测限值的范围从每升亚纳克到低至每纳克低至最低。