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Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
ACS Omega ( IF 3.7 ) Pub Date : 2018-06-20 00:00:00 , DOI: 10.1021/acsomega.8b00240
Ivan Maguire 1 , Jenny Fitzgerald 1 , Brendan Heery 1 , Charles Nwankire 1 , Richard O'Kennedy 1 , Jens Ducrée 1 , Fiona Regan 1
Affiliation  

Globally, the need for “on-site” algal-toxin monitoring has become increasingly urgent due to the amplified demand for fresh-water and for safe, “toxin-free” shellfish and fish stocks. Herein, we describe the first reported, Lab-On-A-Disc (LOAD) based-platform developed to detect microcystin levels in situ, with initial detectability of saxitoxin and domoic acid also reported. Using recombinant antibody technology, the LOAD platform combines immunofluorescence with centrifugally driven microfluidic liquid handling to achieve a next-generation disposable device capable of multianalyte sampling. A low-complexity “LED-photodiode” based optical sensing system was tailor-made for the platform, which allows the fluorescence signal of the toxin-specific reaction to be quantified. This system can rapidly and accurately detect the presence of microcystin-LR, domoic acid, and saxitoxin in 30 min, with a minimum of less than 5 min end-user interaction for maximum reproducibility. This method provides a robust “point of need” diagnostic alternative to the current laborious and costly methods used for qualitative toxin monitoring.

中文翻译:

用于同时检测水中三种藻毒素的新型微流体分析传感平台

在全球范围内,由于对淡水和安全、“无毒”贝类和鱼类种群的需求不断增加,对“现场”藻类毒素监测的需求变得越来越迫切。在此,我们描述了第一个报道的基于光盘实验室 (LOAD) 的平台,该平台开发用于原位检测微囊藻毒素水平,还报道了石房蛤毒素和软骨藻酸的初步检测能力。LOAD 平台利用重组抗体技术,将免疫荧光与离心驱动的微流体液体处理相结合,实现了能够进行多分析物采样的下一代一次性设备。一个低复杂性的基于“LED光电二极管”的光学传感系统是为该平台量身定制的,它可以量化毒素特异性反应的荧光信号。该系统可以在 30 分钟内快速准确地检测微囊藻毒素-LR、软骨藻酸和石房蛤毒素的存在,最终用户交互的时间至少少于 5 分钟,以实现最大的再现性。该方法为当前用于定性毒素监测的费力且昂贵的方法提供了强大的“需求点”诊断替代方案。
更新日期:2018-06-20
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