Triple detection modes for Hg2+ sensing based on a NBD-fluorescent and colorimetric sensor and its potential in cell imaging†,New Journal of Chemistry

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Triple detection modes for Hg2+ sensing based on a NBD-fluorescent and colorimetric sensor and its potential in cell imaging†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-06-20 00:00:00 , DOI: 10.1039/c8nj01915k
Sasiwimon Kraithong _{1,

2,

3,

4,

5} , Rapeepat Sangsuwan _{2,

6,

7,

8} , Nattawan Worawannotai _{2,

3,

9,

10} , Jitnapa Sirirak _{2,

3,

9,

10} , Adisri Charoenpanich _{3,

9,

10,

11} , Patchanita Thamyongkit _{1,

2,

3,

4,

5} , Nantanit Wanichachewa _{2,

3,

9,

10}

Affiliation

A new fluorescent sensor (DiNBD) was successfully synthesized; the sensor consisted of two 7-nitrobenzo-2-oxa-1,3-diazolyl (NBD) units that were covalently attached to 2-(4-(2-aminoethylthio)butylthio)ethanamine by a conventional two-step synthesis. It could serve as a colorimetric and fluorescent sensor for Hg²⁺ in aqueous acetonitrile solutions with high selectivity over other competitive metal ions. Upon Hg²⁺ titration, the sensor exhibited “ON–OFF” fluorescence quenching at 526 nm (λ_ex 458 nm) and “OFF–ON” fluorescence enhancement at 560 nm (λ_ex 530 nm), which were accompanied by a colorimetric change from yellow to red. The Hg²⁺ detection limit of the sensor was 0.7 ppb, which is lower than the permitted level of Hg²⁺ in drinking water regulated by US EPA and WHO. Additionally, the fluorescence quenching and fluorescence enhancement of the sensor in the presence of Hg²⁺ could provide a precise determination of Hg²⁺ concentration due to their self-calibration. Moreover, the DiNBD fluorescent sensor could be used to determine Hg²⁺ in real samples and living cells, which could widen the applications of the developed sensor.

中文翻译：

基于NBD荧光和比色传感器的 Hg ²⁺感测三重检测模式及其在细胞成像中的潜力†

成功合成了一种新型的荧光传感器（DiNBD）。该传感器由两个7-硝基苯并-2-氧杂-1,3-重氮基（NBD）单元组成，这些单元通过常规的两步合成法共价连接到2-（4-（2-氨基乙基硫代）丁基硫代）乙胺上。它可以作为乙腈水溶液中Hg ²⁺的比色和荧光传感器，对其他竞争性金属离子具有高选择性。当汞²⁺滴定，传感器表现出“ON-OFF”荧光猝灭在526纳米（λ _EX 458纳米）和“OFF-ON”的荧光增强在560nm（λ _EX 530纳米），其伴随着色度变化从黄色到红色。汞²⁺传感器的检出限为0.7 ppb，低于美国EPA和WHO规定的饮用水中Hg ²⁺的允许水平。此外，由于Hg ²⁺的自校准，传感器在Hg ²⁺存在下的荧光猝灭和荧光增强可以提供Hg ²⁺浓度的精确测定。此外，DiNBD荧光传感器可用于测定实际样品和活细胞中的Hg ²⁺，这可能会扩展已开发传感器的应用范围。

更新日期：2018-06-20

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