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Identification and Quantitation of Water-Soluble Synthetic Colors in Foods by Liquid Chromatography/Ultraviolet–Visible Method Development and Validation
ACS Omega ( IF 3.7 ) Pub Date : 2018-06-19 00:00:00 , DOI: 10.1021/acsomega.8b00761 Julien Brazeau 1
ACS Omega ( IF 3.7 ) Pub Date : 2018-06-19 00:00:00 , DOI: 10.1021/acsomega.8b00761 Julien Brazeau 1
Affiliation
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A simple and sensitive liquid chromatography method of analysis has been developed and validated for the simultaneous quantitative determination of food colors in a broad range of foods. The method of analysis applies specific extraction solutions to obtain optimal color extraction. The extraction solutions are composed of different proportions of methanol and ammonium acetate, as the ion-pairing agent. Analysis was performed on reverse-phase C18 Poroshell column with ammonium acetate, methanol, acetonitrile, and acetone gradient elution as the mobile phases. Multiple-specific wavelengths were used to monitor color additives in the visible range to provide higher sensitivity and expanded scope needed for a large number of analytes. All 27 color compounds showed good linearity with regression coefficients predominantly above 0.990. The limit of detection and limit of quantitation values ranged from 0.10 to 0.43 and from 0.34 to 1.45 μg/g, respectively. The precision of the method ranged from 1.4 to 15.9%, while recoveries averaged 72% across all food commodities tested. The method of analysis offers convenience and adequate sensitivity for the analysis of a wide variety of food matrices containing a broad range of colors.
中文翻译：
通过液相色谱/紫外-可见光方法开发和验证对食品中的水溶性合成色素进行鉴定和定量
一种简单而灵敏的液相色谱分析方法已经开发出来并得到验证，可用于同时定量测定多种食品中的食品颜色。分析方法应用特定的提取解决方案以获得最佳的颜色提取。萃取液由不同比例的甲醇和作为离子对剂的醋酸铵组成。采用反相 C18 Poroshell 柱，以醋酸铵、甲醇、乙腈、丙酮梯度洗脱为流动相。使用多个特定波长来监测可见光范围内的颜色添加剂，以提供大量分析物所需的更高灵敏度和扩展范围。所有 27 种颜色化合物均表现出良好的线性，回归系数主要高于 0.990。检测限和定量限值分别为0.10至0.43和0.34至1.45 μg/g。该方法的精确度范围为 1.4% 至 15.9%，而所有测试的食品商品的平均回收率为 72%。该分析方法为分析含有多种颜色的各种食品基质提供了便利和足够的灵敏度。
更新日期：2018-06-19
中文翻译：
通过液相色谱/紫外-可见光方法开发和验证对食品中的水溶性合成色素进行鉴定和定量
一种简单而灵敏的液相色谱分析方法已经开发出来并得到验证，可用于同时定量测定多种食品中的食品颜色。分析方法应用特定的提取解决方案以获得最佳的颜色提取。萃取液由不同比例的甲醇和作为离子对剂的醋酸铵组成。采用反相 C18 Poroshell 柱，以醋酸铵、甲醇、乙腈、丙酮梯度洗脱为流动相。使用多个特定波长来监测可见光范围内的颜色添加剂，以提供大量分析物所需的更高灵敏度和扩展范围。所有 27 种颜色化合物均表现出良好的线性，回归系数主要高于 0.990。检测限和定量限值分别为0.10至0.43和0.34至1.45 μg/g。该方法的精确度范围为 1.4% 至 15.9%，而所有测试的食品商品的平均回收率为 72%。该分析方法为分析含有多种颜色的各种食品基质提供了便利和足够的灵敏度。
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