Exploration of the Relationship between Intestinal Colostrum or Milk, and Serum Metabolites in Neonatal Calves by Metabolomics Analysis,Journal of Agricultural and Food Chemistry

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Exploration of the Relationship between Intestinal Colostrum or Milk, and Serum Metabolites in Neonatal Calves by Metabolomics Analysis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-19 00:00:00 , DOI: 10.1021/acs.jafc.8b01621
Yunxia Qi ₁ , Xiaowei Zhao ₁ , Dongwei Huang ₁ , Xiaocheng Pan ₁ , Yongxin Yang ₁ , Huiling Zhao ₁ , Han Hu ₂ , Guanglong Cheng ₁

Affiliation

In contrast to colostral immunoglobulins, changes in metabolite composition of ingested colostrum in the gut have received little attention. Here, we characterized the metabolite profiles of colostrum and milk, ingested colostrum and milk, and serum of neonatal calves by liquid chromatography tandem-mass spectrometry and gas chromatography-mass spectrometry metabolomics approaches. Colostrum and milk underwent similar changes in metabolite profiles in the gut after being ingested. These changes were characterized by increases in methionine, glutamate, thymine, and phosphorylcholine. After ingestion, colostrum concentrations of several metabolites, such as γ-aminobutyric acid, glutamate, cinnamic acid, and thymine increased, whereas concentrations of d-ribose, and arginine decreased. These increases and decreases occurred in a time-dependent manner and were associated with alanine, aspartate, glutamate, and pyrimidine metabolism, and valine, leucine, and isoleucine biosynthesis, respectively. Meanwhile, similar changes in serum metabolites were also observed in neonatal calves fed colostrum, which implies that colostrum metabolites are transported across the small intestine and into the bloodstream. In addition, several metabolites of ingested milk were detected in the gut, and were also transferred to the bloodstream. These metabolites were related to phenylalanine, tyrosine, tryptophan, valine, leucine, and isoleucine biosynthesis, the citrate cycle, and histidine metabolism. These findings reveal that the serum metabolome of neonatal calves’ changes as a result of ingesting colostrum, which can provide health-related benefits in early life.

中文翻译：

代谢组学分析探讨初乳或牛奶与新生犊牛血清代谢产物之间的关系

与初乳免疫球蛋白相反，肠道中摄入的初乳的代谢物组成变化很少受到关注。在这里，我们通过液相色谱-串联质谱和气相色谱-质谱代谢组学方法对初乳和牛奶，摄入的初乳和牛奶以及新生牛血清的代谢产物进行了表征。初乳和牛奶被摄入后，肠道中的代谢产物特征也发生了类似的变化。这些变化的特征在于蛋氨酸，谷氨酸，胸腺嘧啶和磷酸胆碱的增加。摄入后，牛初乳中几种代谢物（例如γ-氨基丁酸，谷氨酸，肉桂酸和胸腺嘧啶）的浓度增加，而d的浓度增加。 -核糖和精氨酸减少。这些增加和减少以时间依赖性方式发生，并分别与丙氨酸，天冬氨酸，谷氨酸和嘧啶代谢以及缬氨酸，亮氨酸和异亮氨酸的生物合成有关。同时，在初乳喂养的初乳犊牛中，血清代谢产物也发生了类似的变化，这意味着初乳代谢产物穿过小肠进入血液。另外，在肠道中检测到几种摄入牛奶的代谢物，这些代谢物也转移到了血液中。这些代谢物与苯丙氨酸，酪氨酸，色氨酸，缬氨酸，亮氨酸和异亮氨酸的生物合成，柠檬酸盐循环和组氨酸代谢有关。这些发现表明，由于摄取初乳，新生儿犊牛的血清代谢组发生了变化，

更新日期：2018-06-19

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